Cancer biomarker discovery for cholangiocarcinoma: the high-throughput approaches.
Cancer biomarker discovery for cholangiocarcinoma: the high-throughput approaches.
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DOI：
10.1002/jhbp.68
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发表时间：
2014-06
影响因子：
3
通讯作者：
Wongkham, Chaisiri
中科院分区：
文献类型：
--
作者：
Silsirivanit, Atit;Sawanyawisuth, Kanlayanee;Riggins, Gregory J.;Wongkham, Chaisiri
胆管癌（CCA）在早期很难诊断，大多数肿瘤在手术或其他治疗无效的晚期才被发现。许多先进的技术被应用于诊断CCA;然而，大多数都是昂贵的，并且具有不同程度的准确性。一个侵入性较小和简单的程序，如血清标志物将是显着的临床效益的诊断，监测和预测CCA患者的结果。“组学”技术的最新进展为建立与疾病相关的生物标志物提供了绝佳的机会。在这篇综述中，从蛋白质组学和糖组学研究中获得的潜在生物标志物进行了评估。采用双向差示凝胶电泳-液相色谱-串联质谱联用技术，从患者标本中发现了几种蛋白质标记物（2D-DIGE/LC-MS-MS），基质辅助激光解吸/电离-飞行时间质谱（MALDI-TOF-MS）、表面增强激光解吸/电离（SELDI）-TOF-MS和毛细管电泳（CE）-MS，使用凝集素辅助、单克隆抗体辅助或特异性靶向策略鉴定新报道的CCA相关糖生物标志物。碳水化合物结合凝集素和核心蛋白结合mAb之间的组合显着增加了CCA糖生物标志物的检测值。寻找特异、敏感的分子标记用于群体筛选是值得探讨的问题。这可能导致早期诊断和改善结果。这些生物标志物功能的进一步研究也是有价值的，以便更好地了解肿瘤生物学，并将其用作未来治疗剂的靶点。
Cholangiocarcinoma (CCA) is difficult to diagnose at an early stage and most tumors are detected at late stage where surgery or other therapy is ineffective. Many advanced techniques are applied to diagnose CCA; however, most are expensive and have varying degrees of accuracy. A less invasive and simpler procedure such as serum markers would be of substantial clinical benefit for diagnosis, monitoring, and predicting outcome for CCA patients. Recent advances in “Omics” technologies offer remarkable opportunities for establishment of biomarker-related to diseases. In this review, the potential biomarkers obtained from proteomics and glycomic studies are evaluated. Several protein markers were discovered from patient specimen, using two dimensional-differential gel electrophoresis couple with liquid chromatography tandem mass spectrometry (2D-DIGE/LC-MS-MS), matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), surface enhanced laser desorption/ionization (SELDI)-TOF-MS and capillary electrophoresis (CE)-MS, etc. Newly reported CCA-associated glycobiomarkers were identified using lectin-assisted, monoclonal antibody-assisted or specific-target strategies. The combination between carbohydrate binding-lectin and core protein-binding mAb significantly increased the values for detection of the glyco-biomarkers for CCA. Searching for specific and sensitive molecular markers to be used for population screening is worth being evaluated. This could lead to earlier diagnosis and improve outcome. Further investigation of those biomarker functions is also of value in order to better understand the tumor biology and use them as targets for future therapeutic agents.
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影响因子：
4.4
作者：
Kristiansen, Troels Zakarias;Harsha, H. C.;Gronborg, Mads;Maitra, Anirban;Pandey, Akhilesh
通讯作者：
Pandey, Akhilesh
DOI：
10.1002/prca.201200134
发表时间：
2013-10
期刊：
Proteomics. Clinical applications
影响因子：
--
作者：
Nyalwidhe JO;Betesh LR;Powers TW;Jones EE;White KY;Burch TC;Brooks J;Watson MT;Lance RS;Troyer DA;Semmes OJ;Mehta A;Drake RR
通讯作者：
Drake RR
影响因子：
--
作者：
Darby, Ian A.;Vuillier-Devillers, Karine;Desmouliere, Alexis
通讯作者：
Desmouliere, Alexis
影响因子：
13.5
作者：
Jinawath, Natini;Chamgramol, Yaovalux;Nakamura, Yusuke
通讯作者：
Nakamura, Yusuke
影响因子：
24.5
作者：
Metzger, Jochen;Negm, Ahmed A.;Lankisch, Tim O.
通讯作者：
Lankisch, Tim O.