Sensitivity enhancement of a grating-based surface plasmon-coupled emission (SPCE) bionsor chip using gold thickness.

Sensitivity enhancement of a grating-based surface plasmon-coupled emission (SPCE) bionsor chip using gold thickness.
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DOI:
10.1016/j.cplett.2013.10.081
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发表时间:
2014-01-20
影响因子:
2.8
通讯作者:
Lynes, Michael A.
Lynes, Michael A.
中科院分区:
化学4区
文献类型:
--
作者:
Yuk, Jong Seol;Guignon, Ernest F.;Lynes, Michael A.

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我们描述了一种新的方法来提高基于光栅的表面等离子体耦合发射(SPCE)传感器的灵敏度,通过增加在这个系统中使用的金属膜的厚度。基于光栅的SPR光谱的计算的光学性质的光栅深度和黄金厚度的显着影响。在短波长和现场测量(水溶液条件下分析)下,可以获得更高的角灵敏度。我们使用固定在SPCE传感器芯片上的Alexa Fluor 647标记的抗小鼠IgG证实了SPCE响应的预测增强。光栅耦合SPCE传感器芯片可以作为一个有用的工具,用于高内容的化学和生物分子相互作用的分析。
We describe a novel approach to enhance the sensitivity of a grating-based surface plasmon-coupled emission (SPCE) sensor by increasing the thickness of the metal film used in this system. The calculated optical properties of grating-based SPR spectra were significantly affected by both grating depth and by gold thickness. Higher angular sensitivity could be achieved at short wavelengths and under in situ measurement (analysis under aqueous condition). We confirmed the predicated enhancements of SPCE response using Alexa Fluor 647-labeled anti-mouse IgG immobilized on the SPCE sensor chips. Grating-coupled SPCE sensor chips can be used as a useful tool for high contents analysis of chemical and biomolecular interactions.
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