Identification and expression of the Actinobacillus actinomycetemcomitans leukotoxin gene.
Identification and expression of the Actinobacillus actinomycetemcomitans leukotoxin gene.
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伴放线放线杆菌白毒素基因的鉴定和表达。
DOI：
10.1016/0006-291x(89)92431-5
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发表时间：
1989
影响因子：
3.1
通讯作者：
Gibson,CW
中科院分区：
文献类型：
--
作者：
Lally,ET;Kieba,IR;Demuth,DR;Rosenbloom,J;Golub,EE;Taichman,NS;Gibson,CW
口腔细菌伴放线放线杆菌产生的白细胞毒素与青少年牙周炎的发病有关。为了阐明白细胞毒素的结构，对白细胞毒素基因进行了分子克隆。A.用Sau 3AI部分消化伴放线菌基因组DNA，将0.5 ~ 5.0个酶对片段连接到质粒载体pENN-vrf的BamHI位点上，构建了伴放线菌JP 2菌株。转化为E. coliRR 1（λ cI 857）中，筛选出产A.用多克隆抗体对放线菌伴放线菌毒素进行了初步研究。鉴定了6个免疫反应性克隆。克隆表达的蛋白质范围为21-80千道尔顿，并选择产生最大蛋白质的命名为pII-2的克隆用于进一步研究。从固定的pII-2蛋白洗脱的抗体也识别nativeA。放线菌共生放线菌毒素分子表明这两种分子共享至少一个表位。DNA序列分析表明，克隆A之间存在显著的氨基酸序列同源性区域。放线菌伴溶血素和其他两种溶细胞素大肠杆菌α-溶血素和巴氏杆菌溶血白细胞毒素，表明可能存在一个具有共同杀伤机制但靶细胞特异性不同的溶细胞素家族。
The leukotoxin produced by the oral bacteriumActinobacillus actinomycetemcomitanshas been implicated in the pathogenesis of juvenile periodontitis. In order to elucidate the structure of the leukotoxin, molecular cloning of the leukotoxin gene was carried out. A DNA library ofA. actinomycetemcomitans, strain JP2, was constructed by partial digestion of genomic DNA withSau3AI and ligation of 0.5 to 5.0 kilobase pair fragments into theBamHI site of the plasmid vector pENN-vrf. After transformation intoE. coliRR1(λcI857), the clones were screened for the production ofA. actinomycetemcomitansleukotoxin with polyclonal antibody. Six immunoreactive clones were identified. The clones expressed proteins which ranged from 21–80 kilodaltons, and the clone designated pII-2, producing the largest protein was selected for further study. Antibodies eluted from immobilized pII-2 protein also recognized the nativeA. actinomycetemcomitansleukotoxin molecule indicating that both molecules shared at least one epitope. DNA sequence analysis demonstrated that there are regions of significant amino acid sequence homology between the clonedA. actinomycetemcomitansleukotoxin and two other cytolysins,Escherichia coliα-hemolysin andPasteurella haemolyticaleukotoxin, suggesting that a family of cytolysins may exist which share a common mechanism of killing but vary in their target cell specificity.
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灵长类动物伴放线放线菌白毒素生物学的比较研究。
影响因子：
--
作者：
Taichman,NS;Simpson,DL;Sakurada,S;Cranfield,M;DiRienzo,J;Slots,J
通讯作者：
Slots,J
影响因子：
3.1
作者：
TAICHMAN, NS;DEAN, RT;SANDERSON, CJ
通讯作者：
SANDERSON, CJ
伴放线放线杆菌对猴血白细胞的细胞病变作用。
影响因子：
3.5
作者：
Taichman,NS;Shenker,BJ;Tsai,CC;Glickman,LT;Baehni,PC;Stevens,R;Hammond,BF
通讯作者：
Hammond,BF
BIONET电子网络
影响因子：
64.8
作者：
D. Kristofferson
通讯作者：
D. Kristofferson
DOI：
10.1016/0735-0651(84)90049-9
发表时间：
1984-01-01
期刊：
Gene Analysis Techniques
影响因子：
--
作者：
Johnson, D. A.;Gautsch, J. W.;Elder, J. H.
通讯作者：
Elder, J. H.