Host-specific transcriptomic pattern of Trichoderma virens during interaction with maize or tomato roots.
Host-specific transcriptomic pattern of Trichoderma virens during interaction with maize or tomato roots.
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DOI：
10.1186/s12864-014-1208-3
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发表时间：
2015-01-22
期刊：
影响因子：
4.4
通讯作者：
Horwitz BA
中科院分区：
文献类型：
--
作者：
Morán-Diez ME;Trushina N;Lamdan NL;Rosenfelder L;Mukherjee PK;Kenerley CM;Horwitz BA
木霉属真菌直接拮抗土传真菌病原体，并且越来越多的物种因其在农业植物病原体生物防治方面的潜力而受到研究。有些物种还定殖于植物根部，促进系统抗性。木霉与根部的相互作用存在于多种植物物种中，包括单子叶植物和双子叶植物。为了验证这种有益相互作用中真菌伙伴的基因表达受到植物调节的假设，将绿木霉与玉米或番茄在水培系统中共培养，从而与根部相互作用。通过在由预测的蛋白质编码基因模型设计的 11645 个独特寡核苷酸微阵列上进行杂交，将单独的 T. virens 转录组与真菌接种的番茄或玉米根进行比较。 210 个基因的转录水平通过与根的相互作用进行调节。几乎所有的都被上调。糖苷水解酶和转运蛋白在与根共培养诱导的转录物中具有很高的代表性。在其中一个或两个宿主植物上调的基因中，有 35 个基因在玉米和番茄之间的表达水平存在显着差异。其中十种在与番茄根共培养的真菌中的表达高于与玉米根的共培养。这些基因的平均转录水平在番茄上比在玉米上高 1.9 倍，在大多数番茄特异性基因上则高 60.9 倍。其他 25 个宿主特异性转录本在与玉米共培养中的表达比与番茄共培养时更强。这些基因的平均转录水平在玉米上比在番茄上高 2.5 至 196 倍。基于绿木霉作为生物防治剂的相关作用，这项研究提供了对其以宿主特异性方式与植物串扰的更好了解。差异表达的基因编码属于多个功能类别的蛋白质，包括酶、转运蛋白和小分泌蛋白。其中，糖苷水解酶和转运蛋白因其丰富而突出，表明在外根层定植期间宿主细胞壁代谢的重要因素。宿主特异性基因表达可能有助于 T. virens 在多种植物的根部定植的能力。本文的在线版本 (doi:10.1186/s12864-014-1208-3) 包含补充材料，可供授权用户使用。
Members of the fungal genus Trichoderma directly antagonize soil-borne fungal pathogens, and an increasing number of species are studied for their potential in biocontrol of plant pathogens in agriculture. Some species also colonize plant roots, promoting systemic resistance. The Trichoderma-root interaction is hosted by a wide range of plant species, including monocots and dicots. To test the hypothesis that gene expression by the fungal partner in this beneficial interaction is modulated by the plant, Trichoderma virens was co-cultured with maize or tomato in a hydroponic system allowing interaction with the roots. The transcriptomes for T. virens alone were compared with fungus-inoculated tomato or maize roots by hybridization on microarrays of 11645 unique oligonucleotides designed from the predicted protein-coding gene models. Transcript levels of 210 genes were modulated by interaction with roots. Almost all were up-regulated. Glycoside hydrolases and transporters were highly represented among transcripts induced by co-culture with roots. Of the genes up-regulated on either or both host plants, 35 differed significantly in their expression levels between maize and tomato. Ten of these were expressed higher in the fungus in co-culture with tomato roots than with maize. Average transcript levels for these genes ranged from 1.9 fold higher on tomato than on maize to 60.9 fold for the most tomato-specific gene. The other 25 host-specific transcripts were expressed more strongly in co-culture with maize than with tomato. Average transcript levels for these genes were 2.5 to 196 fold higher on maize than on tomato. Based on the relevant role of Trichoderma virens as a biological control agent this study provides a better knowledge of its crosstalk with plants in a host-specific manner. The differentially expressed genes encode proteins belonging to several functional classes including enzymes, transporters and small secreted proteins. Among them, glycoside hydrolases and transporters are highlighted by their abundance and suggest an important factor in the metabolism of host cell walls during colonization of the outer root layers. Host-specific gene expression may contribute to the ability of T. virens to colonize the roots of a wide range of plant species. The online version of this article (doi:10.1186/s12864-014-1208-3) contains supplementary material, which is available to authorized users.
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影响因子：
12.3
作者：
Kubicek CP;Herrera-Estrella A;Seidl-Seiboth V;Martinez DA;Druzhinina IS;Thon M;Zeilinger S;Casas-Flores S;Horwitz BA;Mukherjee PK;Mukherjee M;Kredics L;Alcaraz LD;Aerts A;Antal Z;Atanasova L;Cervantes-Badillo MG;Challacombe J;Chertkov O;McCluskey K;Coulpier F;Deshpande N;von Döhren H;Ebbole DJ;Esquivel-Naranjo EU;Fekete E;Flipphi M;Glaser F;Gómez-Rodríguez EY;Gruber S;Han C;Henrissat B;Hermosa R;Hernández-Oñate M;Karaffa L;Kosti I;Le Crom S;Lindquist E;Lucas S;Lübeck M;Lübeck PS;Margeot A;Metz B;Misra M;Nevalainen H;Omann M;Packer N;Perrone G;Uresti-Rivera EE;Salamov A;Schmoll M;Seiboth B;Shapiro H;Sukno S;Tamayo-Ramos JA;Tisch D;Wiest A;Wilkinson HH;Zhang M;Coutinho PM;Kenerley CM;Monte E;Baker SE;Grigoriev IV
通讯作者：
Grigoriev IV
影响因子：
8.7
作者：
Babu, A. Giridhar;Shim, Jaehong;Oh, Byung-Taek
通讯作者：
Oh, Byung-Taek
影响因子：
7.4
作者：
Angel Contreras-Cornejo, Hexon;Macias-Rodriguez, Lourdes;Lopez-Bucio, Jose
通讯作者：
Lopez-Bucio, Jose
DOI：
10.1073/pnas.1301653110
发表时间：
2013-08-20
影响因子：
11.1
作者：
Lahrmann, Urs;Ding, Yi;Zuccaro, Alga
通讯作者：
Zuccaro, Alga
影响因子：
9.2
作者：
Kloppholz, Silke;Kuhn, Hannah;Requena, Natalia
通讯作者：
Requena, Natalia