The biocompatibility of porous vs non-porous bone cements: a new methodological approach.
The biocompatibility of porous vs non-porous bone cements: a new methodological approach.
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DOI：
10.4081/ejh.2014.2255
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发表时间：
2014-06-23
期刊：
影响因子：
--
通讯作者：
Magnan B
中科院分区：
文献类型：
--
作者：
Dall'Oca C;Maluta T;Cavani F;Morbioli GP;Bernardi P;Sbarbati A;Degl'Innocenti D;Magnan B
复合骨水泥已被证明具有生物相容性、生物活性、良好的机械性能和与骨结合的能力。尽管这些有趣的特征，在动物模型的体内研究仍然是不完整的，超微结构的数据是缺乏的。新的超微结构数据的获取受到组织学样本制备方法的不确定性的阻碍，这是由于使用的树脂会熔化骨水泥成分中存在的甲基丙烯酸酯。研制了一种新型多孔丙烯酸骨水泥，该骨水泥由聚甲基丙烯酸甲酯（PMMA）和β-磷酸三钙（p-TCP）组成，并在动物模型上进行了测试。将骨水泥植入8只新西兰白色兔股骨内，处死前观察8周。采用LR白色树脂浸润法制备组织学标本，并进行X线、组织学和扫描电镜观察。作为对照，选择临床手术中常用的丙烯酸标准骨水泥。放射学、超微结构和组织学检查发现新型多孔骨水泥具有良好的生物相容性。骨水泥样本内新生骨组织的生长证明了高度骨整合。未检测到局部或全身毒性体征。目前的工作表明，基于LR白色树脂的使用，拟定的生物相容性评价程序可以对多孔和无孔骨水泥的生物相容性进行全面和客观的评估。
Composite cements have been shown to be biocompatible, bioactive, with good mechanical properties and capability to bind to the bone. Despite these interesting characteristic, in vivo studies on animal models are still incomplete and ultrastructural data are lacking. The acquisition of new ultrastructural data is hampered by uncertainties in the methods of preparation of histological samples due to the use of resins that melt methacrylate present in bone cement composition. A new porous acrylic cement composed of polymethyl-metacrylate (PMMA) and β-tricalcium-phosphate (p-TCP) was developed and tested on an animal model. The cement was implanted in femurs of 8 New Zealand White rabbits, which were observed for 8 weeks before their sacrifice. Histological samples were prepared with an infiltration process of LR white resin and then the specimens were studied by X-rays, histology and scanning electron microscopy (SEM). As a control, an acrylic standard cement, commonly used in clinical procedures, was chosen. Radiographic ultrastructural and histological exams have allowed finding an excellent biocompatibility of the new porous cement. The high degree of osteointegration was demonstrated by growth of neo-created bone tissue inside the cement sample. Local or systemic toxicity signs were not detected. The present work shows that the proposed procedure for the evaluation of biocompatibility, based on the use of LR white resin allows to make a thorough and objective assessment of the biocompatibility of porous and non-porous bone cements.
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影响因子：
9.7
作者：
Wang, Xiupeng;Ye, Jiandong;Wang, Yingjun
通讯作者：
Wang, Yingjun
影响因子：
4.9
作者：
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DOI：
10.4081/ejh.2010.e48
发表时间：
2010-11-25
期刊：
European journal of histochemistry : EJH
影响因子：
--
作者：
Sbarbati A;Accorsi D;Benati D;Marchetti L;Orsini G;Rigotti G;Panettiere P
通讯作者：
Panettiere P
DOI：
10.2106/00004623-198365050-00001
发表时间：
1983-01-01
影响因子：
5.3
作者：
GOLDRING, SR;SCHILLER, AL;HARRIS, WH
通讯作者：
HARRIS, WH