Production of organic acids by probiotic lactobacilli can be used to reduce pathogen load in poultry.
Production of organic acids by probiotic lactobacilli can be used to reduce pathogen load in poultry.
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DOI：
10.1371/journal.pone.0043928
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Konkel ME
中科院分区：
文献类型：
--
作者：
Neal-McKinney JM;Lu X;Duong T;Larson CL;Call DR;Shah DH;Konkel ME
益生菌乳杆菌可以用来减少致病菌在食用动物中的定植，从而降低消费者患食源性疾病的风险。作为一个模型系统，我们研究了乳杆菌在体外抑制空肠弯曲菌生长和减少在肉鸡体内定植的机制。乳杆菌减少病原体的可能机制包括：1)刺激适应性免疫；2)改变盲肠微生物群；3)产生抑制性代谢物，如有机酸。乳杆菌在体外产生的乳酸浓度足以杀死空肠弯曲菌。根据生物光子学的结果，我们确定乳酸杆菌产生的乳酸破坏了空肠弯曲菌的细胞膜。利用傅立叶变换红外光谱(FT-IR)和拉曼光谱技术获得的光谱特征被用来准确预测细菌的生存能力，并根据乳酸处理来区分空肠弯曲菌。共培养的空肠弯曲菌和乳杆菌的FT-IR光谱特征表明，在空肠弯曲菌被杀死之前，其代谢以乳杆菌为主。根据我们的结果，未来竞争排除战略的发展应该包括对有机酸生产的评估。
Probiotic Lactobacillus can be used to reduce the colonization of pathogenic bacteria in food animals, and therefore reduce the risk of foodborne illness to consumers. As a model system, we examined the mechanism of protection conferred by Lactobacillus species to inhibit C. jejuni growth in vitro and reduce colonization in broiler chickens. Possible mechanisms for the reduction of pathogens by lactobacilli include: 1) stimulation of adaptive immunity; 2) alteration of the cecal microbiome; and, 3) production of inhibitory metabolites, such as organic acids. The Lactobacillus species produced lactic acid at concentrations sufficient to kill C. jejuni in vitro. We determined that lactic acid produced by Lactobacillus disrupted the membrane of C. jejuni, as judged by biophotonics. The spectral features obtained using Fourier-transform infrared (FT-IR) and Raman spectroscopy techniques were used to accurately predict bacterial viability and differentiate C. jejuni samples according to lactic acid treatment. FT-IR spectral features of C. jejuni and Lactobacillus grown in co-culture revealed that the metabolism was dominated by Lactobacillus prior to the killing of C. jejuni. Based on our results, the development of future competitive exclusion strategies should include the evaluation of organic acid production.
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影响因子：
2
作者：
Ding, W;Wang, HF;Griffiths, MW
通讯作者：
Griffiths, MW
影响因子：
4.4
作者：
Lu, JR;Idris, U;Lee, MD
通讯作者：
Lee, MD
DOI：
10.1128/cdli.12.12.1387-1392.2005
发表时间：
2005-12-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Haghighi, HR;Gong, JH;Sharif, S
通讯作者：
Sharif, S
影响因子：
3.5
作者：
Al-Qadiri, H. M.;Lin, M.;Rasco, B. A.
通讯作者：
Rasco, B. A.
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM