Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.
Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.
复制标题
DOI：
10.1007/s00253-014-5732-5
复制
发表时间：
2014-06
影响因子：
5
通讯作者：
Schwab, Helmut
中科院分区：
文献类型：
--
作者：
Ahmad, Mudassar;Hirz, Melanie;Pichler, Harald;Schwab, Helmut
巴斯德毕赤酵母是一种成熟的蛋白质表达宿主，主要应用于生物制药和工业酶的生产。这种甲基营养型酵母是一种杰出的生产系统，因为它能生长到非常高的细胞密度，因为它有强大的和严格调控的启动子，并且可以选择在细胞内和以分泌方式每升培养物生产克量的重组蛋白。然而，并非每种目的蛋白质都在巴斯德毕赤酵母中产生或由巴斯德毕赤酵母分泌至如此高的滴度。通常，蛋白质产量明显较低，特别是如果表达的复杂蛋白质是异源寡聚体、膜连接或易于蛋白水解降解。最近几年特别富有成效，因为在改善这种复杂蛋白质的表达方面的许多活动集中在蛋白质工程或蛋白质表达宿主巴斯德毕赤酵母的工程上。本文综述了巴斯德毕赤酵母中蛋白质表达的常用工具，并重点介绍了表达载体设计、宿主菌工程和高水平表达菌株筛选等方面的新进展。膜蛋白表达中的断裂蛋白与巴斯德毕赤酵母衍生蛋白的许多商业应用一起讨论。
Pichia pastoris is an established protein expression host mainly applied for the production of biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished production system for its growth to very high cell densities, for the available strong and tightly regulated promoters, and for the options to produce gram amounts of recombinant protein per litre of culture both intracellularly and in secretory fashion. However, not every protein of interest is produced in or secreted by P. pastoris to such high titres. Frequently, protein yields are clearly lower, particularly if complex proteins are expressed that are hetero-oligomers, membrane-attached or prone to proteolytic degradation. The last few years have been particularly fruitful because of numerous activities in improving the expression of such complex proteins with a focus on either protein engineering or on engineering the protein expression host P. pastoris. This review refers to established tools in protein expression in P. pastoris and highlights novel developments in the areas of expression vector design, host strain engineering and screening for high-level expression strains. Breakthroughs in membrane protein expression are discussed alongside numerous commercial applications of P. pastoris derived proteins.
登录
查看更多内容
影响因子：
6.4
作者：
Aw R;Polizzi KM
通讯作者：
Polizzi KM
影响因子：
5
作者：
Ahn, Jungoh;Hong, Jiyeon;Lee, Hongweon
通讯作者：
Lee, Hongweon
影响因子：
4.4
作者：
Ahn, Jungoh;Hong, Jiyeon;Lee, Hongweon
通讯作者：
Lee, Hongweon
影响因子：
46.9
作者：
De Schutter, Kristof;Lin, Yao-Cheng;Callewaert, Nico
通讯作者：
Callewaert, Nico
影响因子：
2.6
作者：
de Almeida, JRM;de Moraes, LMP;Torres, FAG
通讯作者：
Torres, FAG