Distribution of melanopsin positive neurons in pigmented and albino mice: evidence for melanopsin interneurons in the mouse retina.
Distribution of melanopsin positive neurons in pigmented and albino mice: evidence for melanopsin interneurons in the mouse retina.
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DOI：
10.3389/fnana.2014.00131
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发表时间：
2014
影响因子：
2.9
通讯作者：
Vidal-Sanz M
中科院分区：
文献类型：
--
作者：
Valiente-Soriano FJ;García-Ayuso D;Ortín-Martínez A;Jiménez-López M;Galindo-Romero C;Villegas-Pérez MP;Agudo-Barriuso M;Vugler AA;Vidal-Sanz M
在这里，我们研究了固有光敏视网膜神经节细胞（ipRGCs）的群体在成年色素和白化小鼠。我们的数据显示，尽管色素小鼠（C57Bl/6）和白化小鼠（Swiss）具有相似的iprgc总数，但它们的分布略有不同：色素小鼠的iprgc在颞视网膜中更丰富，而白化小鼠的iprgc在上视网膜中更丰富。在这两种菌株中，ipRGCs都位于视网膜周围，位于Brn3a+RGC密度较低的区域。这两种菌株在内核层（INL）也含有移位的iprgc (d- iprgc)，占色素小鼠iprgc总数的14%，白化小鼠iprgc总数的5%。来自上collicli的追踪显示，98%（色素）和97%（白化）的ipRGCs被逆行标记，而黑视素和Brn3a的双重免疫检测证实，小鼠中很少有ipRGCs表达这种转录因子。令人惊讶的是，将逆行示踪剂应用于视神经（ON）标记出所有iprgc，除了d- iprgc亚群（分别在色素和白化患者中分别为14%和28%）和位于视网膜纤毛边缘带（CMZ）的黑视素阳性细胞。在CMZ中，20%（着色）和24%（白化）的黑视素阳性细胞未被示踪剂标记，我们认为这可能是因为它们无法将轴突送入ON。因此，这项研究为哺乳动物视网膜中存在黑视素中间神经元提供了第一个证据。
Here we have studied the population of intrinsically photosensitive retinal ganglion cells (ipRGCs) in adult pigmented and albino mice. Our data show that although pigmented (C57Bl/6) and albino (Swiss) mice have a similar total number of ipRGCs, their distribution is slightly different: while in pigmented mice ipRGCs are more abundant in the temporal retina, in albinos the ipRGCs are more abundant in superior retina. In both strains, ipRGCs are located in the retinal periphery, in the areas of lower Brn3a+RGC density. Both strains also contain displaced ipRGCs (d-ipRGCs) in the inner nuclear layer (INL) that account for 14% of total ipRGCs in pigmented mice and 5% in albinos. Tracing from both superior colliculli shows that 98% (pigmented) and 97% (albino) of the total ipRGCs, become retrogradely labeled, while double immunodetection of melanopsin and Brn3a confirms that few ipRGCs express this transcription factor in mice. Rather surprisingly, application of a retrograde tracer to the optic nerve (ON) labels all ipRGCs, except for a sub-population of the d-ipRGCs (14% in pigmented and 28% in albino, respectively) and melanopsin positive cells residing in the ciliary marginal zone (CMZ) of the retina. In the CMZ, between 20% (pigmented) and 24% (albino) of the melanopsin positive cells are unlabeled by the tracer and we suggest that this may be because they fail to send an axon into the ON. As such, this study provides the first evidence for a population of melanopsin interneurons in the mammalian retina.
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影响因子：
9.8
作者：
Brown TM;Gias C;Hatori M;Keding SR;Semo M;Coffey PJ;Gigg J;Piggins HD;Panda S;Lucas RJ
通讯作者：
Lucas RJ
影响因子：
25
作者：
Comoli, E;Coizet, V;Redgrave, P
通讯作者：
Redgrave, P
影响因子：
3.4
作者：
Galindo-Romero, C.;Jimenez-Lopez, M.;Vidal-Sanz, M.
通讯作者：
Vidal-Sanz, M.
影响因子：
4.4
作者：
Gonzalez-Menendez, Irene;Contreras, Felipe;Garcia-Fernandez, Jose M.
通讯作者：
Garcia-Fernandez, Jose M.
影响因子：
4.4
作者：
Esquiva, Gema;Lax, Pedro;Cuenca, Nicolas
通讯作者：
Cuenca, Nicolas