Variation in Pleiotropic Hub Gene Expression Is Associated with Interspecific Differences in Head Shape and Eye Size in Drosophila.
Variation in Pleiotropic Hub Gene Expression Is Associated with Interspecific Differences in Head Shape and Eye Size in Drosophila.
复制标题
DOI：
10.1093/molbev/msaa335
复制
发表时间：
2021-05-04
影响因子：
10.7
通讯作者：
Posnien N
中科院分区：
文献类型：
--
作者：
Buchberger E;Bilen A;Ayaz S;Salamanca D;Matas de Las Heras C;Niksic A;Almudi I;Torres-Oliva M;Casares F;Posnien N
揭示在自然界中观察到的惊人的形态多样性背后的机制是生物学的一个重大挑战。已经确定热点基因的重复突变导致形态特征的重复进化，例如身体色素沉着或结构的获得和丧失。然而，到目前为止，热点基因是否有助于器官大小和形状的自然变异仍然难以捉摸。由于果蝇头部形态的自然变异很普遍，我们研究了两种密切相关的果蝇复眼大小和头部形状差异的分子和发育基础。我们显示物种之间的视网膜分化的进展的差异，我们应用比较转录组学和染色质可及性数据，以确定加塔转录因子帕尼尔（Pnr）与这些差异的中心因素。虽然Pnr的遗传操作影响了背头发育的多个方面，但自然变异的影响仅限于表型空间的一个子集。我们目前的数据表明，这种发育限制是由pnr及其辅因子u形（ush）的表达的共同进化引起的。我们建议，自然变化的表达或功能的高度连接的发育调节与多效性功能是形态进化的主要驱动力，我们讨论了基因调控网络进化的影响。与以前的研究结果相比，我们的数据强烈表明，进化热点并不是果蝇眼睛大小和头部形状重复进化的唯一贡献者。
Revealing the mechanisms underlying the breathtaking morphological diversity observed in nature is a major challenge in Biology. It has been established that recurrent mutations in hotspot genes cause the repeated evolution of morphological traits, such as body pigmentation or the gain and loss of structures. To date, however, it remains elusive whether hotspot genes contribute to natural variation in the size and shape of organs. As natural variation in head morphology is pervasive in Drosophila, we studied the molecular and developmental basis of differences in compound eye size and head shape in two closely related Drosophila species. We show differences in the progression of retinal differentiation between species and we applied comparative transcriptomics and chromatin accessibility data to identify the GATA transcription factor Pannier (Pnr) as central factor associated with these differences. Although the genetic manipulation of Pnr affected multiple aspects of dorsal head development, the effect of natural variation is restricted to a subset of the phenotypic space. We present data suggesting that this developmental constraint is caused by the coevolution of expression of pnr and its cofactor u-shaped (ush). We propose that natural variation in expression or function of highly connected developmental regulators with pleiotropic functions is a major driver for morphological evolution and we discuss implications on gene regulatory network evolution. In comparison to previous findings, our data strongly suggest that evolutionary hotspots are not the only contributors to the repeated evolution of eye size and head shape in Drosophila.
登录
查看更多内容
影响因子：
64.5
作者：
Boyle EA;Li YI;Pritchard JK
通讯作者：
Pritchard JK
影响因子：
10.5
作者：
Bessa, J;Gebelein, B;Mann, RS
通讯作者：
Mann, RS
影响因子：
4.6
作者：
Baker, Luke R.;Weasner, Bonnie M.;Kumar, Justin P.
通讯作者：
Kumar, Justin P.
影响因子：
2.7
作者：
Bhattacharya, Abhishek;Baker, Nicholas E.
通讯作者：
Baker, Nicholas E.
影响因子：
56.9
作者：
Barabási, AL;Albert, R
通讯作者：
Albert, R