A modified silent substitution electroretinography protocol to separate photoreceptor subclass function in lightly sedated dogs.
A modified silent substitution electroretinography protocol to separate photoreceptor subclass function in lightly sedated dogs.
复制标题
修改的无声替代电试镜方案，可在轻度镇静的狗中分离光感受器亚类功能。
DOI：
10.1111/vop.12847
复制
发表时间：
2021-01
影响因子：
1.6
通讯作者：
Mowat FM
中科院分区：
文献类型：
--
作者：
Wise EN;Foster ML;Kremers J;Mowat FM
先前发表的一项研究使用在全身麻醉下进行的无声替代视网膜电图（ERG）成功地从犬视杆细胞、长/中波长（L/M）视锥细胞和短波长（S）视锥细胞中分离出光感受器反应。我们假设在镇静状态下犬的反应相似，并且适用于临床患者的缩减方案可以有效地分离所有三种感光细胞亚型的反应。将三只正常成年目的饲养的比格犬（2只雌性，1只雄性）在黑暗中适应1小时。正弦波颜色刺激使用Ganzfeld圆顶中的LED递送。麻醉下的ERG方案如先前发表的那样进行;镇静ERG方案在3天洗脱期后进行。给予静脉镇静（右美托咪定1.25 mcg/kg，布托啡诺0.1 mg/kg）进行镇静。采用双因素重复测量ANOVA和线性回归进行统计分析。在麻醉和镇静状态下，视杆细胞反应在低频（4 - 12Hz）达到峰值，L/M-视锥反应在高频（32 - 38Hz）达到峰值，S-视锥反应在低频（4 - 12Hz）达到峰值。在镇静和麻醉状态下，诱发最大反应的频率相似，但镇静状态下的视杆振幅显著高于麻醉状态（P <0.001）。我们提出了一种临床上适用的方法，以一贯隔离杆，锥亚类功能镇静狗。这可能允许详细评估视杆细胞或视锥细胞亚类缺陷的临床患者的光感受器功能，而不需要全身麻醉或延长适应时间。
A previously published study successfully isolated photoreceptor responses from canine rods, long/medium wavelength (L/M) cones and short wavelength (S) cones using silent substitution electroretinography (ERG) performed under general anesthesia. We hypothesized that responses would be similar in dogs under sedation and that a curtailed protocol suitable for use in clinical patients could effectively isolate responses from all three photoreceptor subtypes. Three normal adult purpose-bred beagles (2 females,1 male) Dogs were dark adapted for 1 hour. Sine wave color stimuli were delivered using LEDs in a Ganzfeld dome. The ERG protocol under anesthesia was performed as previously published; sedated ERG protocols were performed after a 3 day washout period. Intravenous sedation (dexmedetomidine 1.25mcg/kg, butorphanol 0.1mg/kg) was administered for sedation. Statistical analysis was performed using two-way repeated measures ANOVA and linear regression. In both anesthetized and sedated animals, rod-derived responses peaked at low frequency (4–12Hz), L/M-cone responses peaked at high frequency (32–38Hz), S-cone responses peaked at low frequency (4–12Hz). The frequencies eliciting maximal responses were similar in sedated and anesthetized protocols, although rod amplitudes were significantly higher in the sedated protocols compared with anesthetized (P < 0.001). We present a clinically applicable method to consistently isolate rod, and cone subclass function in sedated dogs. This may allow detailed evaluation of photoreceptor function in clinical patients with rod or cone subclass deficits without the need for general anesthesia or protracted adaptation times.
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DOI：
10.1364/josaa.29.00a306
发表时间：
2012-02-01
影响因子：
1.9
作者：
Kremers, Jan;Pangeni, Gobinda
通讯作者：
Pangeni, Gobinda
影响因子：
1
作者：
Freeman, Kate S.;Good, Kathryn L.;Ofri, Ron
通讯作者：
Ofri, Ron
影响因子：
1.9
作者：
NEITZ, J;GEIST, T;JACOBS, GH
通讯作者：
JACOBS, GH
影响因子：
1.4
作者：
Kong, H;Gouras, P
通讯作者：
Gouras, P
影响因子：
1.4
作者：
Jeong, Man Bok;Narfstroem, Kristina;Seo, Kang Moon
通讯作者：
Seo, Kang Moon