Birth of a pathway for sulfur metabolism in early amniote evolution.
Birth of a pathway for sulfur metabolism in early amniote evolution.
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DOI：
10.1038/s41559-020-1232-4
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发表时间：
2020-09
影响因子：
16.8
通讯作者：
Percudani R
中科院分区：
文献类型：
--
作者：
Malatesta M;Mori G;Acquotti D;Campanini B;Peracchi A;Antin PB;Percudani R
在羊膜动物中，爬行动物和哺乳动物适应陆地生活的方式不同。众所周知，陆地化需要脊椎动物代谢的适应性变化，特别是氮排泄模式的适应性变化。然而，目前的范式是，对陆地上生命的代谢适应并不涉及重新合成酶途径，而是重新利用现有途径。在这里，通过比较不同羊膜动物中吡脲醛磷酸依赖酶（PLPome）的库存，我们在计算机上确定了鸡胚胎中存在的硫代谢途径，而在哺乳动物中不存在。半胱氨酸裂解酶（CL）含有血红素和PLP辅助因子，将半胱氨酸和亚硫酸盐转化为半胱氨酸和硫化氢。一种特殊的半胱氨酸脱羧酶（CAD）产生牛磺酸，而硫化氢通过半胱硫氨酸-合成酶（CBS）循环转化为半胱氨酸。这个反应序列使得在胚胎发育期间在卵子中形成磺化氨基酸而不以减少硫为代价。该通路起源于大约3亿年前的原始爬行动物，通过CBS复制、CL新功能化和CAD共选择。我们的研究结果表明，对陆地生活的适应涉及代谢途径的创新，并揭示了这种创新在羊膜进化中产生的分子机制。
Among amniotes, reptiles and mammals are differently adapted to terrestrial life. It is well appreciated that terrestrialization required adaptive changes of vertebrate metabolism, particularly in the mode of nitrogen excretion. However, the current paradigm is that metabolic adaptation to life on land did not involve synthesis of enzymatic pathways de novo but repurposing of existing ones. Here, by comparing the inventory of pyridoxal phosphate-dependent enzymes (PLPome) in different amniotes, we identify in silico a pathway for sulfur metabolism present in chick embryos but not in mammals. Cysteine lyase (CL) contains heme and PLP cofactors and converts cysteine and sulfite into cysteic acid and hydrogen sulfide. A specific cysteic acid decarboxylase (CAD) produces taurine while hydrogen sulfide is recycled into cysteine by cystathionine beta-synthase (CBS). This reaction sequence enables the formation of sulfonated amino acids during embryo development in the egg at no cost of reduced sulfur. The pathway originated around 300 million years ago in a proto-reptile by CBS duplication, CL neofunctionalization, and CAD co-option. Our findings indicate that adaptation to terrestrial life involved innovations in metabolic pathways and reveal the molecular mechanisms by which such innovations arose in amniote evolution.
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影响因子：
4.8
作者：
Chiku, Taurai;Padovani, Dominique;Banerjee, Ruma
通讯作者：
Banerjee, Ruma
影响因子：
16.8
作者：
Ford, David P.;Benson, Roger B. J.
通讯作者：
Benson, Roger B. J.
影响因子：
14.9
作者：
Antin PB;Yatskievych TA;Davey S;Darnell DK
通讯作者：
Darnell DK
影响因子：
4.4
作者：
Burt DW;Carrë W;Fell M;Law AS;Antin PB;Maglott DR;Weber JA;Schmidt CJ;Burgess SC;McCarthy FM
通讯作者：
McCarthy FM
DOI：
10.1016/0006-3002(57)90102-6
发表时间：
1957-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
CHAPEVILLE, F;FROMAGEOT, P
通讯作者：
FROMAGEOT, P