Effect of Tea Tree Oil on the Expression of Genes Involved in the Innate Immune System in Goat Rumen Epithelial Cells.
Effect of Tea Tree Oil on the Expression of Genes Involved in the Innate Immune System in Goat Rumen Epithelial Cells.
复制标题
茶树油对山羊瘤胃上皮细胞天然免疫系统相关基因表达的影响。
DOI：
10.3390/ani11082460
复制
发表时间：
2021-08-21
期刊：
影响因子：
--
通讯作者：
Zhan K
中科院分区：
文献类型：
--
作者：
Hu Z;Lin M;Ma X;Zhao G;Zhan K
亚急性瘤胃酸中毒（SARA）往往造成重大损失的商业农场。SARA主要由革兰氏阴性菌裂解产生的内毒素（LPS）引起，其引起炎症反应。为了减轻LPS介导的炎症反应，提高动物生产性能是重要的。茶树油（TTO）是一种植物提取物，具有良好的杀菌和抗炎作用。本研究结果表明，LPS能显著诱导山羊瘤胃上皮细胞（GRECs）的炎症反应，而TTO能显著减轻LPS介导的GRECs的炎症反应。因此，它可能有助于治疗SARA。在亚急性瘤胃酸中毒（SARA）中，瘤胃上皮经常受到内毒素（LPS）的攻击，内毒素是由死亡的革兰氏阴性菌裂解而引起的。而瘤胃上皮天然免疫系统对感染有积极的反应。以往的研究表明，茶树油（TTO）具有良好的杀菌和抗炎作用。因此，本研究的目的是研究TTO对LPS诱导的山羊瘤胃上皮细胞（GRECs）炎症因子相关基因表达的影响。本研究表明，从山羊瘤胃组织中分离的瘤胃上皮细胞可以在0.25%胰蛋白酶中长期体外培养。这些细胞通过表达细胞角蛋白18、单羧酸转运体4（MCT 4）、Na[+]/H[+]氢交换体1（NHE 1）、推定阴离子转运体1（PAT 1）、vH+ ATP酶B亚基（vH+ ATP酶）和阴离子交换体2（AE 2）鉴定为上皮细胞。与未处理对照组相比，当使用LPS时，IL-1β、IL-6、TNF-α、TLR-2、NF-κB、CXCL 6和CXCL 8基因的mRNA表达显著增加。此外，LPS组IL-1β、IL-6、TNF-α、TLR-2、NF-κB、CXCL 8、CXCL 6和干扰素诱导蛋白3（IFIT 3）基因的mRNA表达也显著高于0.05%TTO组。但与1 μg/mL LPS组相比，LPS和0.05%TTO组IL-1β、IL-6、TNF-α、TLR-2、CXCL 6和IFIT 3基因的表达显著降低。这些结果表明，TTO可以抑制LPS诱导的炎症细胞因子在GRECs中的表达。
Subacute rumen acidosis (SARA) often causes significant losses on commercial farms. SARA is mainly caused by endotoxin (LPS) produced by the lysis of Gram-negative bacteria, which causes an inflammatory response. To alleviate the inflammatory response mediated by LPS, it is important to improve animal production performance. Tea tree oil (TTO) is a plant extract that possesses good bactericidal and anti-inflammatory effects. According to this study, LPS can significantly induce inflammatory responses in goat rumen epithelial cells (GRECs), while the addition of TTO could markedly mitigate inflammatory responses mediated by LPS in GRECs. Therefore, it may be useful for the treatment of SARA. In subacute rumen acidosis (SARA), the rumen epithelium is frequently attacked by endotoxin (LPS), which is caused by the lysis of dead Gram-negative bacteria. However, the rumen epithelium innate immune system can actively respond to the infection. Previous studies have demonstrated that tea tree oil (TTO) has good bactericidal and anti-inflammatory effects. Therefore, the aim of this study was to investigate the effect of TTO on the expression of genes involved in the inflammatory cytokines in goat rumen epithelial cells (GRECs) triggered by LPS. Our study shows that rumen epithelial cells isolated from goat rumen tissue can be cultured in vitro in 0.25% trypsin for a long time. These cells were identified as epithelial cells by the expression of cytokeratin 18, monocarboxylate transporter 4 (MCT4), Na[+]/H[+] hydrogen exchanger 1 (NHE1), putative anion transporter 1 (PAT1), vH+ ATPase B subunit (vH+ ATPase), and anion exchanger 2 (AE2). The mRNA expression of IL-1β, IL-6, TNF-α, TLR-2, NF-κB, CXCL6 and CXCL8 genes was significantly increased when LPS was used compared to untreated controls. In addition, mRNA expression of IL-1β, IL-6, TNF-α, TLR-2, NF-κB, CXCL8, CXCL6 and interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) genes was also significantly higher in the LPS group compared to the 0.05% TTO group. However, the expression of IL-1β, IL-6, TNF-α, TLR-2, CXCL6 and IFIT3 genes was significantly lower in the LPS and 0.05% TTO group compared to the 1 μg/mL LPS group. These results suggest that TTO can inhibit LPS-induced inflammatory cytokines expression in GRECs.
登录
查看更多内容
干扰素诱导趋化因子转录水平升高与系统性红斑狼疮患者疾病活动和器官损伤的关联。
影响因子：
4.9
作者：
Fu, Qiong;Chen, Xiaoqing;Cui, Huijuan;Guo, Yanzhi;Chen, Jing;Shen, Nan;Bao, Chunde
通讯作者：
Bao, Chunde
影响因子：
8.5
作者：
Ehmann, UK;DeVries, JT;Omary, MB
通讯作者：
Omary, MB
影响因子：
3.3
作者：
Caldefie-Chézet, F;Guerry, M;Guillot, J
通讯作者：
Guillot, J
影响因子：
4.6
作者：
Abe, S;Maruyama, N;Yamaguchi, H
通讯作者：
Yamaguchi, H
影响因子：
6.7
作者：
Brand, C;Townley, SL;Hart, PH
通讯作者：
Hart, PH