Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast.
Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast.
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裂变酵母SWI/SNF和RSC络合物显示出与萌芽酵母的组成和功能差异。
DOI：
10.1038/nsmb.1452
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发表时间：
2008-08
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
--
作者：
SWI/SNF染色质重塑复合体在转录和其他染色质相关过程中起着至关重要的作用。对酿酒酵母中这一类成员SWI/SNF和RSC的分析，极大地有助于我们对这些复合体的理解。为了了解SWI/SNF和RSC在进化上遥远的生物体中的体内功能，我们在裂殖酵母中鉴定了这些复合体。虽然核心成分在两个酵母之间是保守的，但S.pombe SWI/SNF和RSC的组成与它们的酿酒酵母不同，在某些方面更类似于后生动物复合体。此外，一些保守的蛋白质，包括肌动蛋白样蛋白，在两种酵母菌的生存要求方面有明显的不同。最后，表型和微阵列分析确定了对SWI/SNF和RSC在转录上的广泛需求，包括强有力的证据表明SWI/SNF直接抑制铁运输基因。
SWI/SNF chromatin-remodeling complexes have crucial roles in transcription and other chromatin-related processes. The analysis of the two members of this class in Saccharomyces cerevisiae, SWI/SNF and RSC, has heavily contributed to our understanding of these complexes. To understand the in vivo functions of SWI/SNF and RSC in an evolutionarily distant organism, we have characterized these complexes in Schizosaccharomyces pombe. Although core components are conserved between the two yeasts, the compositions of S. pombe SWI/SNF and RSC differ from their S. cerevisiae counterparts and in some ways are more similar to metazoan complexes. Furthermore, several of the conserved proteins, including actin-like proteins, are markedly different between the two yeasts with respect to their requirement for viability. Finally, phenotypic and microarray analyses identified widespread requirements for SWI/SNF and RSC on transcription including strong evidence that SWI/SNF directly represses iron-transport genes.
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影响因子：
3.5
作者：
Labbe, Simon;Pelletier, Benoit;Mercier, Alexandre
通讯作者：
Mercier, Alexandre
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5.3
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LAURENT, BC;YANG, XL;CARLSON, M
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CARLSON, M
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