Dimeric lipoprotein lipase is bound to triglyceride-rich plasma lipoproteins.
Dimeric lipoprotein lipase is bound to triglyceride-rich plasma lipoproteins.
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二聚脂蛋白脂肪酶与富含甘油三酯的血浆脂蛋白结合。
DOI：
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发表时间：
1996
影响因子：
6.5
通讯作者：
John D. Brunzell
中科院分区：
文献类型：
--
作者：
Albert 0 Zambon;Ivo Schmidt;Ulrike Beisiegel;John D. Brunzell
脂蛋白脂肪酶能分解乳糜粒和极低密度脂蛋白中富含甘油三酯的核心部分。在体外，它也是脂蛋白与低密度脂蛋白受体相关蛋白结合的配体，并可能在受体介导的富含甘油三酯的脂蛋白的清除中发挥核心作用。本研究的目的是确定酶在预肝素血浆中结合到哪个脂蛋白亚类上，以及当通过肝素注射释放到血浆中时。四氢脂抑素是一种有效的丝氨酸脂酶抑制剂，用于阻断脂解活性，从而防止由于体外脂解而引起的血浆脂蛋白的变化。为分析脂蛋白脂酶二聚体在不同脂蛋白类别中的分布规律，采用特异性酶联免疫吸附试验，对5例甘油三酯为69~522 mg/dl的受试者肝素前、后血浆进行凝胶过滤。当脂解活性没有被抑制时，脂蛋白脂肪酶二聚体与低密度脂蛋白和高密度脂蛋白一起洗脱，重复了几个研究小组之前获得的结果。然而，在用四氢脂抑素处理的肝素前和肝素后样本中，发现大多数二聚体酶与极低密度脂蛋白颗粒有关。总之，在肝素前和肝素后的样品中，当体外脂解活性被抑制时，大多数脂蛋白脂酶二聚体与极低密度脂蛋白相关，这支持了在体内，脂蛋白脂酶可能影响受体介导的这些颗粒的清除的假设。此外，这表明脂蛋白脂肪酶和富含胆固醇的脂蛋白之间的联系可能是一种体外现象，因为缺乏对脂解活性的抑制。
Lipoprotein lipase hydrolyzes the triglyceride-rich core of chylomicrons and very low density lipoproteins. It is also a ligand, in vitro, for binding of lipoproteins to the low density lipoprotein receptor-related protein and may play a central role in the receptor-mediated removal of triglyceride-rich lipoproteins. The aim of the present study was to determine to which lipoprotein subclass the enzyme is bound in preheparin plasma and when released into plasma by heparin injection. Tetrahydrolipstatin, a potent inhibitor of serine lipases, was used to block lipolytic activity, thereby preventing changes in plasma lipoproteins due to ex vivo lipolysis. To analyze the distribution pattern of lipoprotein lipase dimers among lipoprotein classes, a specific ELISA was used and gel filtration was performed in pre- and postheparin plasma from five subjects with triglyceride ranging from 69 to 522 mg/dl. When lipolytic activity was not inhibited, lipoprotein lipase dimers eluted in association with low and high density lipoproteins, reproducing results previously obtained by several groups of investigators. However, in pre- and postheparin samples treated with tetrahydrolipstatin, most of the dimeric enzyme was found associated with very low density lipoprotein particles. In conclusion in pre- and postheparin samples most of the lipoprotein lipase dimers are associated with very low density lipoproteins when ex vivo lipolytic activity is inhibited, which supports the hypothesis that, in vivo, lipoprotein lipase may affect the receptor-mediated removal of these particles. Moreover, it suggests that the association between lipoprotein lipase and cholesterol-rich lipoproteins might be an ex vivo phenomenon due to lack of inhibition of lipolytic activity.
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血浆脂蛋白与肝素后脂肪酶活性的关联。
DOI：
10.1172/jci112744
发表时间：
1986
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Goldberg,IJ;Kandel,JJ;Blum,CB;Ginsberg,HN
通讯作者：
Ginsberg,HN
DOI：
10.1152/ajpendo.1985.249.1.e107
发表时间：
1985-07
期刊：
The American journal of physiology
影响因子：
--
作者：
P. Iverius;J. Brunzell
通讯作者：
P. Iverius;J. Brunzell
分析获取血浆以测量游离脂肪酸水平的技术。
影响因子：
6.5
作者：
Zambon,A;Hashimoto,SI;Brunzell,JD
通讯作者：
Brunzell,JD
影响因子：
56.9
作者：
WION, KL;KIRCHGESSNER, TG;LAWN, RM
通讯作者：
LAWN, RM
血浆脂蛋白和游离脂肪酸释放内皮细胞脂蛋白脂肪酶。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Saxena,U;Witte,LD;Goldberg,IJ
通讯作者：
Goldberg,IJ