Fine-Mapping of a Wild Genomic Region Involved in Pod and Seed Size Reduction on Chromosome A07 in Peanut (Arachis hypogaea L.).
Fine-Mapping of a Wild Genomic Region Involved in Pod and Seed Size Reduction on Chromosome A07 in Peanut (Arachis hypogaea L.).
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DOI：
10.3390/genes11121402
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发表时间：
2020-11-25
期刊：
影响因子：
3.5
通讯作者：
Fonceka D
中科院分区：
文献类型：
--
作者：
Alyr MH;Pallu J;Sambou A;Nguepjop JR;Seye M;Tossim HA;Djiboune YR;Sane D;Rami JF;Fonceka D
果实和种子大小是作物驯化过程中选择的重要产量组成性状。利用栽培品种Fleur11与野生异源四倍体Arachis ipaensis × Arachis duranensis 4x杂交，开发了先进回交数量性状位点（AB-QTL）和染色体片段代换系（CSSL）群体。在AB-QTL群体中，在A07染色体近端检测到荚果和种子大小的主要QTL，间隔约5mb。在CSSL群体中，鉴定出了携带QTL区域的品系12CS_091，其荚果和种子比Fleur11小。本研究采用两步策略对A07染色体上的种子大小QTL区域进行了精细定位。我们开发了新的SSR和SNP标记，以及目标QTL区域的近等基因系（NILs）。通过对2172个F2大群体的基因分型和单标记分析方法，我们首次在两个SSR标记之间的约1mb区域找到了QTL。利用均匀分布在精化QTL区域的9个新SNP标记，对88 F2衍生的490株F3植株进行了基因型分析，筛选出10个NILs。NILs的表型分析和标记/性状关联使我们能够将QTL区域缩小到168.37 kb染色体片段，位于snp Aradu_A07_1148327和Aradu_A07_1316694之间。该区域包含22个预测基因。在这些基因中，Aradu。DN3DB和Aradu。RLZ61分别编码一个转录调控因子无菌无瓣花序样（SAP）和一个F-box喷嚏子（SNE），我们对此特别感兴趣。讨论了这些基因在调节果实和种子大小变化中的作用。这项研究将有助于更好地了解花生驯化过程中靶向的基因。
Fruit and seed size are important yield component traits that have been selected during crop domestication. In previous studies, Advanced Backcross Quantitative Trait Loci (AB-QTL) and Chromosome Segment Substitution Line (CSSL) populations were developed in peanut by crossing the cultivated variety Fleur11 and a synthetic wild allotetraploid (Arachis ipaensis × Arachis duranensis)4x. In the AB-QTL population, a major QTL for pod and seed size was detected in a ~5 Mb interval in the proximal region of chromosome A07. In the CSSL population, the line 12CS_091, which carries the QTL region and that produces smaller pods and seeds than Fleur11, was identified. In this study, we used a two-step strategy to fine-map the seed size QTL region on chromosome A07. We developed new SSR and SNP markers, as well as near-isogenic lines (NILs) in the target QTL region. We first located the QTL in ~1 Mb region between two SSR markers, thanks to the genotyping of a large F2 population of 2172 individuals and a single marker analysis approach. We then used nine new SNP markers evenly distributed in the refined QTL region to genotype 490 F3 plants derived from 88 F2, and we selected 10 NILs. The phenotyping of the NILs and marker/trait association allowed us to narrowing down the QTL region to a 168.37 kb chromosome segment, between the SNPs Aradu_A07_1148327 and Aradu_A07_1316694. This region contains 22 predicted genes. Among these genes, Aradu.DN3DB and Aradu.RLZ61, which encode a transcriptional regulator STERILE APETALA-like (SAP) and an F-box SNEEZY (SNE), respectively, were of particular interest. The function of these genes in regulating the variation of fruit and seed size is discussed. This study will contribute to a better knowledge of genes that have been targeted during peanut domestication.
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