The Effect of Hyperbaric Oxygen Therapy on Markers of Oxidative Stress and the Immune Response in Healthy Volunteers.
The Effect of Hyperbaric Oxygen Therapy on Markers of Oxidative Stress and the Immune Response in Healthy Volunteers.
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DOI：
10.3389/fphys.2022.826163
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发表时间：
2022
影响因子：
4
通讯作者：
Kox M
中科院分区：
文献类型：
--
作者：
de Wolde SD;Hulskes RH;de Jonge SW;Hollmann MW;van Hulst RA;Weenink RP;Kox M
高压氧治疗（HBOT）包括在增加的环境压力下呼吸100%的氧气。有迹象表明，HBOT诱导氧化应激和激活免疫途径。然而，以前对HBOT免疫学效应的研究主要是在体外实验和选定的患者人群中建立的，这限制了普遍性，并增加了合并症和特定患者相关因素的混淆机会。对HBOT的免疫学效应的更多了解将有助于对潜在的新型治疗应用的研究和理解。因此，在这项研究中，我们研究了三个110分钟的HBO-会议与24小时的间隔对免疫参数的健康，年轻，男性志愿者。在第一次和第三次HBOT治疗前后采集血样。我们评估了嗜中性粒细胞活性氧（ROS）的产生，全身氧化应激[血浆丙二醛（MDA）浓度]以及中性粒细胞吞噬活性，肿瘤坏死因子（TNF）、白细胞介素（IL）-6，IL-8和IL-10的血浆浓度，以及TNF，IL-6，和IL-10通过用Toll样受体（TLR）配体脂多糖（TLR 4）和Pam 3Cys（TLR 2）离体刺激的白细胞。我们观察到第二次HBOT后嗜酸性ROS产生和吞噬作用下降，第三次HBOT后持续存在，但MDA浓度无变化。此外，所研究的细胞因子的血浆浓度在所有时间点均未改变，并且离体细胞因子产生也随时间基本上未改变。这些结果表明，在健康志愿者中重复HBOT后没有诱导全身氧化应激或全身炎症反应，但可能表明ROS产生能力和吞噬作用耗尽。
Hyperbaric oxygen therapy (HBOT) consists of breathing 100% oxygen under increased ambient pressure. There are indications that HBOT induces oxidative stress and activates immune pathways. However, previous research on immunological effects of HBOT has mainly been established in in vitro experiments and selected patient populations, limiting generalizability and increasing the chances of confounding by comorbidities and specific patient-related factors. More insight into the immunological effects of HBOT would aid investigation and comprehension of potentially novel treatment applications. Therefore, in this study, we investigated the effects of three 110-min HBOT-sessions with 24-h intervals on immunological parameters in healthy, young, male volunteers. Blood samples were obtained before and after the first and third HBOT sessions. We assessed neutrophilic reactive oxygen species (ROS) production, systemic oxidative stress [plasma malondialdehyde (MDA) concentrations] as well as neutrophil phagocytic activity, plasma concentrations of tumor necrosis factor (TNF), interleukin (IL)-6, IL-8, and IL-10, and production of TNF, IL-6, and IL-10 by leukocytes ex vivo stimulated with the Toll-like receptor (TLR) ligands lipopolysaccharide (TLR4) and Pam3Cys (TLR2). We observed decreased neutrophilic ROS production and phagocytosis following the second HBOT session, which persisted after the third session, but no alterations in MDA concentrations. Furthermore, plasma concentrations of the investigated cytokines were unaltered at all-time points, and ex vivo cytokine production was largely unaltered over time as well. These results indicate no induction of systemic oxidative stress or a systemic inflammatory response after repeated HBOT in healthy volunteers but may suggest exhaustion of ROS generation capacity and phagocytosis.
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DOI：
10.3390/ijerph17207377
发表时间：
2020-10-10
影响因子：
--
作者：
Woo J;Min JH;Lee YH;Roh HT
通讯作者：
Roh HT
影响因子：
6.1
作者：
Almzaiel, Anwar J.;Billington, Richard;Moody, A. John
通讯作者：
Moody, A. John
影响因子：
4.2
作者：
Kox, Matthijs;Vrouwenvelder, Maarten Q.;Hoedemaekers, Cornelia W.
通讯作者：
Hoedemaekers, Cornelia W.
反复高压氧预处理可对接受冠状动脉搭桥手术的患者产生心肌和大脑保护：一项前瞻性、随机、对照临床试验
DOI：
10.1053/j.jvca.2011.06.017
发表时间：
2011-12-01
影响因子：
2.8
作者：
Li, Yang;Dong, Hailong;Xiong, Lize
通讯作者：
Xiong, Lize
影响因子：
0.7
作者：
Matzi, Veronika;Greilberger, Joachim F.;Smolle-Juettner, Freyja-Maria
通讯作者：
Smolle-Juettner, Freyja-Maria