Heat Shock 70 kDa Protein Cognate 3 of Brown Planthopper Is Required for Survival and Suppresses Immune Response in Plants.
Heat Shock 70 kDa Protein Cognate 3 of Brown Planthopper Is Required for Survival and Suppresses Immune Response in Plants.
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褐飞虱热激 70 kDa 蛋白同源 3 是植物生存和抑制免疫反应所必需的
DOI：
10.3390/insects13030299
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发表时间：
2022-03-17
期刊：
影响因子：
3
通讯作者：
Jiang Y
中科院分区：
文献类型：
--
作者：
Yang H;Zhang X;Li H;Ye Y;Li Z;Han X;Hu Y;Zhang C;Jiang Y
褐飞虱（BPH）的热激70 kDa同源蛋白3（NlHSC70 - 3）对褐飞虱的生长发育很重要。然而，它是否也调节植物生理过程仍不清楚。在本研究中，通过比较褐飞虱侵害后日本晴组织的蛋白质图谱鉴定出了NlHSC70 - 3。预测的信号肽以及在唾液腺和卵巢中的高水平转录表明它是一种分泌蛋白。功能分析表明，NlHSC70 - 3是褐飞虱在水稻上生存所必需的。在本氏烟中，NlHSC70 - 3抑制了植物免疫反应，包括flg22诱导的活性氧（ROS）爆发以及防御相关基因的表达。因此，NlHSC70 - 3被褐飞虱分泌到水稻中，并可能干扰植物防御反应以利于其自身生存。
褐飞虱（Nilaparvata lugens）是水稻（Oryza sativa）的单食性害虫，威胁着全球粮食安全。昆虫热激蛋白70 kDa（Hsp70s）在昆虫生长发育中起关键作用，然而它们是否也调节植物生理过程仍不清楚。在本研究中，我们通过液相色谱/质谱（LC/MS）比较褐飞虱侵害后日本晴组织的蛋白质图谱，鉴定出了褐飞虱的热激70 kDa同源蛋白3（NlHSC70 - 3）。NlHSC70 - 3具有预测的信号肽，并且在唾液腺中显示出高转录水平，这进一步证明它在取食过程中被褐飞虱分泌到植物中。通过RNA干扰（RNAi），我们表明NlHSC70 - 3对褐飞虱在水稻上的生存是不可或缺的。最重要的是，NlHSC70 - 3介导了本氏烟中的植物免疫反应，包括细胞死亡、flg22诱导的ROS爆发以及防御相关基因的表达。这些结果表明，NlHSC70 - 3可能作为一种效应子调节植物生理过程，以利于害虫在水稻上生存，这为未来的害虫防治提供了一个新的潜在靶点。
Simple Summary Heat shock 70 kDa protein cognate 3 (NlHSC70-3) of brown planthopper (BPH) is important to the growth and development of BPH. However, if it also regulates plant physiological processes remain unclear. In this study, NlHSC70-3 is identified from a compared protein profile of Nipponbare tissues after BPH infestation. The predicted signal peptide and high levels of transcription in salivary glands and ovaries supported that it is a secreted protein. Function analysis demonstrates that NlHSC70-3 is required for the survival of BPH on rice. Plant immune responses, including flg22-induced ROS bursts and the expression of defense-related genes, are suppressed by NlHSC70-3 in Nicotiana benthamiana. Thus, NlHSC70-3 is secreted into rice by BPH and may interfere with plant defense responses to facilitate its survival. Abstract The brown planthopper (Nilaparvata lugens) is a monophagous pest of rice (Oryza sativa), which threatens food security around the world. Insect Heat shock proteins 70 kDa (Hsp70s) play a key role in insect growth and development, however, if they also modulate the plant physiological processes is still unclear. In this study, we identified the Heat shock 70 kDa protein cognate 3 (NlHSC70-3) of BPH from compared protein profiles of Nipponbare tissues after BPH infestation via LC/MS. NlHSC70-3 has a predicted signal peptide and displays high transcription levels in the salivary glands, which further supported that it is secreted into plants by BPH during the feeding process. Using RNA interference (RNAi), we showed that NlHSC70-3 is indispensable for the survival of BPH on rice. Most importantly, NlHSC70-3 mediates the plant immune responses including cell death, flg22-induced ROS burst and defense-related gene expression in N. benthamiana. These results demonstrate that NlHSC70-3 may function as an effector manipulating plant physiological processes to facilitate pest survival on rice, which provides a new potential target for future pest control.
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DOI：
10.1186/s12284-016-0099-0
发表时间：
2016-12
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