Comparative transcriptome analysis of salivary glands of two populations of rice brown planthopper, Nilaparvata lugens, that differ in virulence.
Comparative transcriptome analysis of salivary glands of two populations of rice brown planthopper, Nilaparvata lugens, that differ in virulence.
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DOI：
10.1371/journal.pone.0079612
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Lou Y
中科院分区：
文献类型：
--
作者：
Ji R;Yu H;Fu Q;Chen H;Ye W;Li S;Lou Y
褐飞虱（Nilaparvata lugens（Stål））是亚洲水稻的一种毁灭性害虫，它可以通过进化新的强毒种群来快速克服水稻的抗性。草食动物唾液在植物-草食动物相互作用中起着重要作用，包括植物防御和草食动物毒力。然而，到目前为止，很少有人知道BPH唾液在分子水平上，特别是其在毒性和BPH-水稻互作中的作用。利用cDNA扩增结合Illumina短读测序技术，对来自水稻品种TN 1（TN 1群体）和Mudgo（M群体）的两个具有不同毒力的BPH群体的唾液腺转录组进行了测序。总共有37，666和38，451个单基因分别从这些人群的唾液腺中产生。当组合时，总共获得了43，312个unigenes，比先前从这些腺体中鉴定的表达序列标签的数量多约18倍。基因本体注释和KEGG分类表明，与代谢，结合和运输相关的基因在唾液腺中显着活跃。共编码352个分泌蛋白基因，其中一些可能在褐飞虱取食和褐飞虱与水稻互作中起重要作用。对两个种群的转录组进行比较分析，发现与“代谢”、“消化吸收”和“唾液分泌”相关的基因可能与毒力相关。此外，67个基因编码推定的分泌蛋白的差异表达的两个群体之间，这表明这些基因可能有助于毒力的变化。这项研究是第一次比较两个BPH人群具有不同的毒力性状的唾液腺转录组，并找到可能与这种差异有关的基因。我们的数据提供了一个丰富的分子资源，为未来的功能研究唾液腺，将有助于阐明BPH的摄食和毒力差异的分子机制。
The brown planthopper (BPH), Nilaparvata lugens (Stål), a destructive rice pest in Asia, can quickly overcome rice resistance by evolving new virulent populations. Herbivore saliva plays an important role in plant–herbivore interactions, including in plant defense and herbivore virulence. However, thus far little is known about BPH saliva at the molecular level, especially its role in virulence and BPH–rice interaction. Using cDNA amplification in combination with Illumina short-read sequencing technology, we sequenced the salivary-gland transcriptomes of two BPH populations with different virulence; the populations were derived from rice variety TN1 (TN1 population) and Mudgo (M population). In total, 37,666 and 38,451 unigenes were generated from the salivary glands of these populations, respectively. When combined, a total of 43,312 unigenes were obtained, about 18 times more than the number of expressed sequence tags previously identified from these glands. Gene ontology annotations and KEGG orthology classifications indicated that genes related to metabolism, binding and transport were significantly active in the salivary glands. A total of 352 genes were predicted to encode secretory proteins, and some might play important roles in BPH feeding and BPH–rice interactions. Comparative analysis of the transcriptomes of the two populations revealed that the genes related to ‘metabolism,’ ‘digestion and absorption,’ and ‘salivary secretion’ might be associated with virulence. Moreover, 67 genes encoding putative secreted proteins were differentially expressed between the two populations, suggesting these genes may contribute to the change in virulence. This study was the first to compare the salivary-gland transcriptomes of two BPH populations having different virulence traits and to find genes that may be related to this difference. Our data provide a rich molecular resource for future functional studies on salivary glands and will be useful for elucidating the molecular mechanisms underlying BPH feeding and virulence differences.
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