Long-Term Storage Stability and Nitric Oxide Release Behavior of (N-Acetyl-S-nitrosopenicillaminyl)-S-nitrosopenicillamine-Incorporated Silicone Rubber Coatings.
Long-Term Storage Stability and Nitric Oxide Release Behavior of (N-Acetyl-S-nitrosopenicillaminyl)-S-nitrosopenicillamine-Incorporated Silicone Rubber Coatings.
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DOI：
10.1021/acsami.2c06712
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发表时间：
2022-07-13
影响因子：
9.5
通讯作者：
Brisbois, Elizabeth J.
中科院分区：
文献类型：
--
作者：
Kumar, Rajnish;Chug, Manjyot Kaur;Brisbois, Elizabeth J.
在生物医学级聚合物基质中物理结合一氧化氮（NO）释放材料以制造抗微生物涂层和装置是经济上可行的方法。然而，实现长期的NO释放与最小或没有从聚合物基质的NO供体浸出仍然是一个具有挑战性的任务。本文将青霉胺二肽NO释放分子（N-乙酰基-S-亚硝基青霉胺）-S-亚硝基青霉胺（SNAP-SNAP）掺入市售生物医学级硅橡胶（SR）中，制成NO释放涂层（SNAP-SNAP/SR）。分析了SNAP-SNAP粉末和SNAP-SNAP/SR涂层在不同温度下的贮存稳定性。具有不同重量%的SNAP-SNAP的SNAP-SNAP/SR涂层显示出长达6周的可调且持续的NO释放。此外，S-亚硝基-N-乙酰青霉胺（SNAP），一个充分开发的NO释放分子，被纳入到生物医学级的有机硅聚合物，以制造NO释放涂层（SNAP/SR）和10重量% SNAP-SNAP/SR和10重量%SNAP/SR的NO释放和S-亚硝基硫醇（RSNO）浸出行为的比较分析进行了研究。有趣的是，10重量% SNAP-SNAP/SR涂层表现出比10重量% SNAP/SR涂层高约36%的NO释放和少4倍的NO供体浸出。此外，10重量%的SNAP-SNAP/SR涂层表现出有前途的抗菌性能对金黄色葡萄球菌和大肠杆菌由于持续释放NO。10重量%的SNAP-SNAP/SR涂层也被发现是生物相容性对NIH 3 T3小鼠成纤维细胞。这些结果证实了SNAP-SNAP在有机硅聚合物基质中的持续稳定性和NO释放特性，并证明了SNAP-SNAP/SR聚合物在制造长期留置生物医学器械和植入物中的潜力，以增强生物相容性并抵抗器械相关感染。
Physical incorporation of nitric oxide (NO) releasing materials in biomedical grade polymer matrices to fabricate antimicrobial coatings and devices is an economically viable process. However, achieving long-term NO release with a minimum or no leaching of the NO donor from the polymer matrix is still a challenging task. Herein, (N-acetyl-S-nitrosopenicillaminyl)-S-nitrosopenicillamine (SNAP-SNAP), a penicillamine dipeptide NO-releasing molecule, is incorporated into a commercially available biomedical grade silicone rubber (SR) to fabricate a NO-releasing coating (SNAP-SNAP/SR). The storage stabilities of the SNAP-SNAP powder and SNAP-SNAP/SR coating were analyzed at different temperatures. The SNAP-SNAP/SR coatings with varying wt % of SNAP-SNAP showed a tunable and sustained NO release for up to 6 weeks. Further, S-nitroso-N-acetylpenicillamine (SNAP), a well-explored NO-releasing molecule, was incorporated into a biomedical grade silicone polymer to fabricate a NO-releasing coating (SNAP/SR) and a comparative analysis of the NO release and S-nitrosothiol (RSNO) leaching behavior of 10 wt % SNAP-SNAP/SR and 10 wt % SNAP/SR was studied. Interestingly, the 10 wt % SNAP-SNAP/SR coatings exhibited ~36% higher NO release and 4 times less leaching of NO donors than the 10 wt % SNAP/SR coatings. Further, the 10 wt % SNAP-SNAP/SR coatings exhibited promising antibacterial properties against Staphylococcus aureus and Escherichia coli due to the persistent release of NO. The 10 wt % SNAP-SNAP/SR coatings were also found to be biocompatible against NIH 3T3 mouse fibroblast cells. These results corroborate the sustained stability and NO-releasing properties of the SNAP-SNAP in a silicone polymer matrix and demonstrate the potential for the SNAP-SNAP/SR polymer in the fabrication of long-term indwelling biomedical devices and implants to enhance biocompatibility and resist device-related infections.
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影响因子：
9.7
作者：
Brisbois EJ;Major TC;Goudie MJ;Bartlett RH;Meyerhoff ME;Handa H
通讯作者：
Handa H
基于 S-亚硝基谷胱甘肽的释放一氧化氮的纳米纤维在生物医学应用中表现出双重抗菌和抗血栓活性。
影响因子：
4.6
作者：
Douglass M;Hopkins S;Pandey R;Singha P;Norman M;Handa H
通讯作者：
Handa H
影响因子：
19
作者：
Jen, Michele C.;Serrano, Maria C.;van Lith, Robert;Ameer, Guillermo A.
通讯作者：
Ameer, Guillermo A.
影响因子：
168.9
作者：
Allegranzi, Benedetta;Nejad, Sepideh Bagheri;Pittet, Didier
通讯作者：
Pittet, Didier
影响因子：
4.7
作者：
Chug, Manjyot Kaur;Feit, Corbin;Brisbois, Elizabeth J.
通讯作者：
Brisbois, Elizabeth J.