Transcriptional homeostasis of a mangrove species, Ceriops tagal, in saline environments, as revealed by microarray analysis.
Transcriptional homeostasis of a mangrove species, Ceriops tagal, in saline environments, as revealed by microarray analysis.
复制标题
微阵列分析揭示了盐环境中红树林物种 Ceriops tagal 的转录稳态
DOI：
10.1371/journal.pone.0036499
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Shi S
中科院分区：
文献类型：
--
作者：
Liang S;Fang L;Zhou R;Tang T;Deng S;Dong S;Huang Y;Zhong C;Shi S
背景转录水平上对环境应激的差异反应在适应中发挥着关键作用。红树林物种构成了潮间带的优势群落，并在海陆交界处形成茂密的森林，尽管与其耐盐生活方式相关的解剖和生理特征已得到很好的表征，但人们对转录表型对其适应这些盐分环境的影响知之甚少。方法和主要发现 我们报告了一系列微阵列实验揭示的真正红树物种 Ceriops tagal 根部的时间过程转录谱。盐胁迫下，C. tagal根中共有432个转录本的表达发生显着变化，其中83个转录本发生2倍以上的变化，并进一步组装成59个unigenes。整体转录在盐胁迫的早期阶段是稳定的，然后随着胁迫持续时间的增加而逐渐失调。重要的是，预测同源基因对的成对比较表明，大多数差异表达基因的转录调控在塔加尔中与盐敏感物种拟南芥中的转录调控高度不同。结论/意义这项工作表明，转录稳态和特异性转录调控是红树根遭受盐休克时的主要事件，这可能有助于建立对盐分环境的适应，从而促进该红树种的耐盐生活方式。此外，通过比较分析确定了适应的候选基因。这项研究为剖析红树林适应潮间带环境的遗传基础奠定了基础。
Background Differential responses to the environmental stresses at the level of transcription play a critical role in adaptation. Mangrove species compose a dominant community in intertidal zones and form dense forests at the sea-land interface, and although the anatomical and physiological features associated with their salt-tolerant lifestyles have been well characterized, little is known about the impact of transcriptional phenotypes on their adaptation to these saline environments. Methodology and Principal findings We report the time-course transcript profiles in the roots of a true mangrove species, Ceriops tagal, as revealed by a series of microarray experiments. The expression of a total of 432 transcripts changed significantly in the roots of C. tagal under salt shock, of which 83 had a more than 2-fold change and were further assembled into 59 unigenes. Global transcription was stable at the early stage of salt stress and then was gradually dysregulated with the increased duration of the stress. Importantly, a pair-wise comparison of predicted homologous gene pairs revealed that the transcriptional regulations of most of the differentially expressed genes were highly divergent in C. tagal from that in salt-sensitive species, Arabidopsis thaliana. Conclusions/Significance This work suggests that transcriptional homeostasis and specific transcriptional regulation are major events in the roots of C. tagal when subjected to salt shock, which could contribute to the establishment of adaptation to saline environments and, thus, facilitate the salt-tolerant lifestyle of this mangrove species. Furthermore, the candidate genes underlying the adaptation were identified through comparative analyses. This study provides a foundation for dissecting the genetic basis of the adaptation of mangroves to intertidal environments.
登录
查看更多内容
NACL胁迫的拟南芥根的全面转录分析揭示了新的反应基因类别。
影响因子：
5.3
作者：
Jiang, Yuanqing;Deyholos, Michael K.
通讯作者：
Deyholos, Michael K.
影响因子：
7.4
作者：
Martinez-Atienza, Juliana;Jiang, Xingyu;Quintero, Francisco J.
通讯作者：
Quintero, Francisco J.
影响因子：
7.4
作者：
NIU, XM;NARASIMHAN, ML;HASEGAWA, PM
通讯作者：
HASEGAWA, PM
影响因子：
1.8
作者：
Aziz, I;Khan, MA
通讯作者：
Khan, MA
影响因子：
3.7
作者：
Lira-Medeiros CF;Parisod C;Fernandes RA;Mata CS;Cardoso MA;Ferreira PC
通讯作者：
Ferreira PC