Chitosan in viscosupplementation: in vivo effect on rabbit subchondral bone.
Chitosan in viscosupplementation: in vivo effect on rabbit subchondral bone.
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DOI：
10.1186/s12891-017-1700-4
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发表时间：
2017-08-15
影响因子：
2.3
通讯作者：
Hoc T
中科院分区：
文献类型：
--
作者：
Rieger R;Boulocher C;Kaderli S;Hoc T
为探讨关节腔内注射壳聚糖(Cs)和透明质酸(HA)对骨关节炎(OA)软骨下骨的影响，在兔早期骨关节炎模型上测量了软骨下骨的微结构参数和骨密度。将真菌来源的重乙酰化Cs添加到HA中的新型杂化水凝胶与高分子量HA商业配方进行了比较。18只兔行单侧前交叉韧带横断术(ACLT)，根据关节内注射化合物的不同分为生理盐水组、透明质酸组和混合组。8个对侧膝关节作为非手术对照(对照组)。在ACLT术后6周进行微型计算机断层扫描，以研究软骨下骨微结构参数和骨密度在骨性关节炎发生的早期阶段。软骨厚度平均值仅生理盐水组较对侧组降低。杂交组与生理盐水组比较，软骨下骨微结构参数(骨小梁厚度和骨小梁体积分数)发生明显变化，软骨下骨板和骨小梁骨密度(骨密度和组织密度)降低。杂交组与HA组相比，软骨下骨微结构参数(软骨板厚度和骨小梁厚度)和骨小梁密度(骨密度和组织密度)均显著降低。结论：与单独使用HA相比，在HA中添加Cs的新型杂化水凝胶可以增强骨形态结构参数和骨密度的变化，导致兔早期实验性骨关节炎模型软骨下骨丢失。
To investigate the effect of intra-articular injection of Chitosan (Cs) added to hyaluronic acid (HA) on subchondral bone during osteoarthritis (OA), microarchitectural parameters and mineral density were measured in a rabbit model of early OA. A novel hybrid hydrogel adding reacetylated Cs of fungal origin to HA was compared to high molecular weight HA commercial formulation. Eighteen rabbits underwent unilateral anterior cruciate ligament transection (ACLT) and were divided into three groups (Saline-group, HA-group and Hybrid-group) depending on the intra-articular injection compound. Eight contralateral knees were used as non-operated controls (Contralateral-group). Micro-computed tomography was performed six weeks post-ACLT to study subchondral bone microarchitectural parameters and mineral density at an early stage of OA development. Cartilage thickness mean value was reduced only in Saline-group compared to Contralateral-group. When the Hybrid-group was compared to Saline-group, subchondral bone microarchitectural parameters (trabecular thickness and trabecular bone volume fraction) were significantly changed; subchondral bone plate and trabecular bone mineral densities (bone mineral density and tissue mineral density) were reduced. When the Hybrid-group was compared to HA-group, subchondral bone microarchitectural parameters (subchondral plate thickness and trabecular thickness) and trabecular bone mineral densities (bone mineral density and tissue mineral density) were significantly decreased. Conclusion: Compared to HA alone, the novel hybrid hydrogel, constituted of Cs added to HA, enhanced microarchitectural parameters and mineral density changes, leading to subchondral bone loss in a rabbit model of early experimental OA.
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影响因子：
1.3
作者：
Patchornik S;Ram E;Ben Shalom N;Nevo Z;Robinson D
通讯作者：
Robinson D
影响因子：
3.9
作者：
Han, Sang-Kuy;Seerattan, Ruth;Herzog, Walter
通讯作者：
Herzog, Walter
影响因子：
2.8
作者：
Hiraoka, Nobuyuki;Takahashi, Kenji A.;Kubo, Toshikazu
通讯作者：
Kubo, Toshikazu
影响因子：
14
作者：
MUZZARELLI, RAA;ZUCCHINI, C;CASTALDINI, C
通讯作者：
CASTALDINI, C
影响因子：
7
作者：
Elmorsy, S.;Funakoshi, T.;Iwasaki, N.
通讯作者：
Iwasaki, N.