Monitoring Histone Methylation (H3K9me3) Changes in Live Cells
Monitoring Histone Methylation (H3K9me3) Changes in Live Cells
复制标题
监测活细胞中组蛋白甲基化 (H3K9me3) 的变化
DOI：
10.1021/acsomega.9b01413
复制
发表时间：
2019
期刊：
影响因子：
4.1
通讯作者：
Yuan, Chongli
中科院分区：
文献类型：
--
作者：
Sánchez, Oscar F.;Mendonca, Agnes;Min, Alan;Liu, Jichang;Yuan, Chongli
H3K9me3（组蛋白H3赖氨酸9的甲基化）是一种表观遗传修饰，作为抑制标记。包括癌症和神经系统疾病在内的几种疾病都与H3K9me3水平的异常变化有关。已经开发了不同的工具来检测和定量细胞中的H3K9me3水平。然而，大多数技术缺乏活细胞兼容性。为了解决这个问题，我们设计了重组蛋白传感器，用于原位探测H3K9me3。发现含有来自HP1a的色域和色影域的异二聚体传感器在识别H3K9me3方面是最佳的，并且具有与商业抗体相似的空间分辨率。与抗体相比，我们的传感器在表征H3K9me3变化方面提供类似的定量准确性，但要求单细胞分辨率。该传感器用于评价H3K9me3对环境化学物质阿特拉津（ATZ）的响应变化。发现ATZ在暴露24小时后导致H3K9me3水平显著降低。它对H3K9me3在细胞群中的分布的影响也被评估，并发现是独特的。我们预见到我们的传感器在多种毒性和药物筛选应用中的应用。
H3K9me3 (methylation of lysine 9 of histone H3) is an epigenetic modification that acts as a repressor mark. Several diseases, including cancers and neurological disorders, have been associated with aberrant changes in H3K9me3 levels. Different tools have been developed to enable detection and quantification of H3K9me3 levels in cells. Most techniques, however, lack live cell compatibility. To address this concern, we have engineered recombinant protein sensors for probing H3K9me3 in situ. A heterodimeric sensor containing a chromodomain and chromo shadow domain from HP1a was found to be optimal in recognizing H3K9me3 and exhibited similar spatial resolution to commercial antibodies. Our sensor offers similar quantitative accuracy in characterizing changes in H3K9me3 compared to antibodies but claims single cell resolution. The sensor was applied to evaluate changes in H3K9me3 responding to environmental chemical atrazine (ATZ). ATZ was found to result in significant reductions in H3K9me3 levels after 24 h of exposure. Its impact on the distribution of H3K9me3 among cell populations was also assessed and found to be distinctive. We foresee the application of our sensors in multiple toxicity and drug-screening applications.
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影响因子：
3.8
作者：
Bisson, M;Hontela, A
通讯作者：
Hontela, A
活细胞中的组蛋白修饰传感器
DOI：
10.1201/b18007-19
发表时间：
2015
期刊：
Cardiologia
影响因子：
--
作者：
H. Kimura;Y. Sato
通讯作者：
Y. Sato
影响因子：
4
作者：
Dialynas, George K.;Terjung, Stefan;Georgatos, Spyros D.
通讯作者：
Georgatos, Spyros D.
阿特拉津和毒死蜱对鲤鱼 (Cyprinus carpio L.) 肝脏、肾脏和鳃 DNA 甲基化的影响。
影响因子：
6.8
作者：
Chao Wang;Ziwei Zhang;Hai;Fuqing Zhao;Liangliang Wang;Xiaolong Wang;H. Xing;Shiwen Xu
通讯作者：
Shiwen Xu
影响因子：
56.9
作者：
Yu, Junying;Vodyanik, Maxim A.;Thomson, James A.
通讯作者：
Thomson, James A.