Hydrogen peroxide can be a plausible biomarker in cyanobacterial bloom treatment.
Hydrogen peroxide can be a plausible biomarker in cyanobacterial bloom treatment.
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DOI：
10.1038/s41598-021-02978-6
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发表时间：
2022-01-07
影响因子：
4.6
通讯作者：
Abeynayaka HDL
中科院分区：
文献类型：
--
作者：
Asaeda T;Rahman M;Abeynayaka HDL
为了建立蓝藻生物量预测模型，在实验室实验中测定了复合胁迫、光抑制和营养耗竭对蓝藻氧化胁迫的影响。在不同的光合作用有效辐射(PAR)强度和不同浓度的磷(P)浓度下，对悬铃草进行了氮素浓度固定的处理。通过测定过氧化氢(H_2O_2)浓度以及过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的抗氧化活性，对样品进行应激测试。H_2O_2浓度从PAR的S−_1降至30µm−_2，然后随着PAR强度的增加而增加。在磷浓度方面，过氧化氢浓度(L−1)一般随着磷浓度的增加而降低。超氧化物歧化酶和过氧化氢酶活性与过氧化氢蛋白−1成正比。细胞外没有检测到H_2O_2浓度表明细胞内有H_2O_2的生物产生，细胞内积累的H_2O_2浓度受H_2O_2浓度蛋白−_1的影响。当PAR的浓度超过30µm−_2和S−_1时，H_2O_2浓度蛋白−_1随着PAR强度的增加有相似的增加趋势，与P~+浓度无关。同时，随着磷浓度的增加，无论PAR强度如何，H_2O_2蛋白−-1均以相似的方式减少。蛋白质含量随着H_2O_2的逐渐增加而降低，最高可达4nmolH_2O_2 mg−_1蛋白质，这为限制蓝藻的生长提供了阈值。根据这些结果，建立了一个经验公式-蛋白质(mg·L−1) = −=192×Log((H_2O_2/蛋白质)/4.1)，式中H_2O_2/蛋白质(nmol·mg−_1) = −_(Nmo1)与总磷浓度P(µg·L·−_1)--用于获得蓝藻的生物量。
The effect of combined stresses, photoinhibition, and nutrient depletion on the oxidative stress of cyanobacteria was measured in laboratory experiments to develop the biomass prediction model. Phormidium ambiguum was exposed to various photosynthetically active radiation (PAR) intensities and phosphorous (P) concentrations with fixed nitrogen concentrations. The samples were subjected to stress assays by detecting the hydrogen peroxide (H2O2) concentration and antioxidant activities of catalase (CAT) and superoxide dismutase (SOD). H2O2 concentrations decreased to 30 µmol m−2 s−1 of PAR, then increased with higher PAR intensities. Regarding P concentrations, H2O2 concentrations (nmol L−1) generally decreased with increasing P concentrations. SOD and CAT activities were proportionate to the H2O2 protein−1. No H2O2 concentrations detected outside cells indicated the biological production of H2O2, and the accumulated H2O2 concentration inside cells was parameterized with H2O2 concentration protein−1. With over 30 µmol m−2 s−1 of PAR, H2O2 concentration protein−1 had a similar increasing trend with PAR intensity, independently of P concentration. Meanwhile, with increasing P concentration, H2O2 protein−1 decreased in a similar pattern regardless of PAR intensity. Protein content decreased with gradually increasing H2O2 up to 4 nmol H2O2 mg−1 protein, which provides a threshold to restrict the growth of cyanobacteria. With these results, an empirical formula—protein (mg L−1) = − 192*Log((H2O2/protein)/4.1), where H2O2/protein (nmol mg−1) = − 0.312*PAR2/(502 + PAR2)*((25/PAR)4 + 1)*Log(P/133,100), as a function of total phosphorus concentration, P (µg L−1)—was developed to obtain the cyanobacteria biomass.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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