Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their "stemness".
Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their "stemness".
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DOI：
10.1371/journal.pone.0011462
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发表时间：
2010-07-07
期刊：
影响因子：
3.7
通讯作者：
Robey PG
中科院分区：
文献类型：
--
作者：
Balakumaran A;Pawelczyk E;Ren J;Sworder B;Chaudhry A;Sabatino M;Stroncek D;Frank JA;Robey PG
超顺磁性氧化铁纳米颗粒(Spion)越来越多地被用于标记人骨髓基质细胞(BMSCs，又称间充质干细胞)，以通过体内MRI和普鲁士蓝(PB)染色后的组织学来监测其命运。通过对骨髓间充质干细胞体外分化的评估，Spion标记似乎是安全的，然而，我们选择解决标记在体内维持骨髓间充质干细胞群体内细胞的“干性”的作用的问题。进行的分析包括集落形成效率、CD146表达、基因表达谱，以及评估免疫低下受者体内骨和骨髓支持间质形成的“金标准”。SPION标记并没有改变这些检测结果。在植入Spion标记或未标记BMSCs的小鼠队列中，可以看到与相邻宿主造血细胞相当丰富的骨骼。发现了PB+脂肪细胞，证明了它们的供体来源，以及PB+周细胞，表明在BMSC群体中干细胞可以自我更新。这项研究证实，Spion标记不会改变骨髓间充质干细胞内干细胞亚群的分化潜力。
Superparamagnetic iron oxide nanoparticles (SPION) are increasingly used to label human bone marrow stromal cells (BMSCs, also called “mesenchymal stem cells”) to monitor their fate by in vivo MRI, and by histology after Prussian blue (PB) staining. SPION-labeling appears to be safe as assessed by in vitro differentiation of BMSCs, however, we chose to resolve the question of the effect of labeling on maintaining the “stemness” of cells within the BMSC population in vivo. Assays performed include colony forming efficiency, CD146 expression, gene expression profiling, and the “gold standard” of evaluating bone and myelosupportive stroma formation in vivo in immuncompromised recipients. SPION-labeling did not alter these assays. Comparable abundant bone with adjoining host hematopoietic cells were seen in cohorts of mice that were implanted with SPION-labeled or unlabeled BMSCs. PB+ adipocytes were noted, demonstrating their donor origin, as well as PB+ pericytes, indicative of self-renewal of the stem cell in the BMSC population. This study confirms that SPION labeling does not alter the differentiation potential of the subset of stem cells within BMSCs.
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DOI：
10.1084/jem.150.4.919
发表时间：
1979-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Westen H;Bainton DF
通讯作者：
Bainton DF
影响因子：
2.9
作者：
Pawelczyk, Edyta;Arbab, Ali S.;Frank, Joseph A.
通讯作者：
Frank, Joseph A.
影响因子：
64.5
作者：
Sacchetti, Benedetto;Funari, Alessia;Bianco, Paolo
通讯作者：
Bianco, Paolo
影响因子：
19.7
作者：
Mankani, MH;Kuznetsov, SA;Robey, PG
通讯作者：
Robey, PG
DOI：
10.1073/pnas.0403918101
发表时间：
2004-07-27
影响因子：
11.1
作者：
Shapiro, EM;Skrtic, S;Koretsky, AP
通讯作者：
Koretsky, AP