Critical media attributes in E-beam sterilization of corneal tissue.
Critical media attributes in E-beam sterilization of corneal tissue.
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角膜组织电子束灭菌中的关键培养基属性。
DOI：
10.1016/j.actbio.2021.10.033
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发表时间：
2022-01-15
影响因子：
9.7
通讯作者：
Chodosh J
中科院分区：
文献类型：
--
作者：
Sharifi S;Sharifi H;Akbari A;Lei F;Dohlman CH;Gonzalez-Andrades M;Guild C;Paschalis EI;Chodosh J
当电离辐射与介质相互作用时，它可以形成活性物质，这些活性物质可以与系统的成分反应，导致生物堵塞的消除和组织的灭菌。了解介质的性质，如极性，对于控制和指导这些活性物种执行所需的反应非常重要。本文利用乙醇作为水的极性修饰剂，制备了一系列相对极性不同的介质，用于25kGy电子束辐照角膜，并研究了辐照介质的极性对角膜性能的影响。角膜组织照射后，对角膜组织的力学性能(拉伸强度和弹性模量、断裂伸长率和压缩弹性模量)、化学性能、光学性能、结构性能、降解性能和生物学特性进行了评价。我们的研究表明，在较低的相对极性介质中照射改善了组织的结构特性，但降低了光学透过率；较高的相对极性降低了角膜的结构和光学特性；而中等相对极性(乙醇浓度=20-30%(v/v))改善了组织的结构特性，而不影响光学特性。无论介质的极性如何，照射都不会对角膜组织的生物相容性产生负面影响。我们的数据显示，吸收的乙醇可以从照射后的角膜中冲洗到对角膜和视网膜细胞无毒的水平。这些发现表明，可以调整照射介质的相对极性，以产生无菌组织，包括角膜移植物，具有特定生物医学应用所需的工程特性。
When ionizing irradiation interacts with a media, it can form reactive species that can react with the constituents of the system, leading to eradication of bioburden and sterilization of the tissue. Understanding the media’s properties such as polarity is important to control and direct those reactive species to perform desired reactions. Using ethanol as a polarity modifier of water, we herein generated a series of media with varying relative polarities for electron beam (E-beam) irradiation of cornea at 25 kGy and studied how the irradiation media’s polarity impacts properties of the cornea. After irradiation of corneal tissues, mechanical (tensile strength and modulus, elongation at break, and compression modulus), chemical, optical, structural, degradation, and biological properties of the corneal tissues were evaluated. Our study showed that irradiation in lower relative polarity media improved structural properties of the tissues yet reduced optical transmission; higher relative polarity reduced structural and optical properties of the cornea; and intermediate relative polarity (ethanol concentrations = 20–30% (v/v)) improved the structural properties, without compromising optical characteristics. Regardless of media polarity, irradiation did not negatively impact the biocompatibility of the corneal tissue. Our data shows that the absorbed ethanol can be flushed from the irradiated cornea to levels that are nontoxic to corneal and retinal cells. These findings suggest that the relative polarity of the irradiation media can be tuned to generate sterilized tissues, including corneal grafts, with engineered properties that are required for specific biomedical applications.
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影响因子：
2.8
作者：
Bakshi, Shaunak K.;Graney, John;Chodosh, James
通讯作者：
Chodosh, James
影响因子：
1.5
作者：
Gut G;Marowska J;Jastrzebska A;Olender E;Kamiński A
通讯作者：
Kamiński A
影响因子：
1.5
作者：
Kaminski, A.;Gut, G.;Zasacka, M.
通讯作者：
Zasacka, M.
影响因子：
2.4
作者：
Cissell, Derek D.;Hu, Jerry C.;Griffiths, Leigh G.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
影响因子：
13.7
作者：
Borderie, Vincent M.;Boelle, Pierre-Yves;Laroche, Laurent
通讯作者：
Laroche, Laurent