Comparative ploidy response to experimental hydrogen peroxide exposure in Atlantic salmon (Salmo salar).
Comparative ploidy response to experimental hydrogen peroxide exposure in Atlantic salmon (Salmo salar).
复制标题
DOI：
10.1016/j.fsi.2018.07.017
复制
发表时间：
2018-10
影响因子：
4.7
通讯作者：
Migaud H
中科院分区：
文献类型：
--
作者：
Chalmers L;Vera LM;Taylor JF;Adams A;Migaud H
虽然对三倍体大西洋鲑鱼的生长、生存、营养以及最近的疾病易感性的研究不断扩大，但总体上仍然缺乏评估三倍体对化学处理的反应的研究。必须表征三倍体对疾病治疗的反应，以验证其商业生产的适用性。本研究旨在调查和比较三倍体和二倍体大西洋鲑鱼在用过氧化氢 (H2O2) 进行实验性处理后的应激和免疫反应。首先进行剂量反应测试以确定二倍体和三倍体大西洋鲑鱼的合适测试剂量。随后，按照商业惯例，将二倍体和三倍体暴露于 H2O2 (1800ppm) 20 分钟，然后测量血糖和乳酸、血浆皮质醇和溶菌酶，以及氧化应激和免疫相关基因的表达。在接触 H2O2 后的前 6 小时内，二倍体和三倍体大西洋鲑鱼的死亡率相当。皮质醇、葡萄糖和乳酸没有受到倍性的显着影响，这表明，从生理上讲，三倍体大西洋鲑鱼能够像二倍体鲑鱼一样应对与 H2O2 暴露相关的压力。 H2O2暴露显着升高二倍体肝脏中cat和sod2的表达以及二倍体鳃中gr、il1β和crp/sap1b的表达，同时显着降低二倍体鳃中saa5和crp/sap1a的表达。在三倍体中，暴露于 H2O2 的鱼肝脏中 cat、hsp70、sod1、saa5、crp/sap1a 和 crp/sap1b 的表达水平显着高于对照鱼。三倍体鳃中 gr、sod1 和 il1β 的表达也因 H2O2 暴露而升高。这项研究代表了 H2O2 暴露对三倍体大西洋鲑鱼影响的第一个实验证据，并继续支持其在商业生产中的应用。这项研究是首次在三倍体大西洋鲑鱼中测试 H2O2。暴露于 H2O2 后，三倍体和二倍体大西洋鲑鱼的皮质醇、葡萄糖和乳酸水平相似。基因表达的倍性差异可能与三倍体细胞中额外的遗传物质有关，但需要进一步研究。
While research into the growth, survival, nutrition and, more recently, disease susceptibility of triploid Atlantic salmon has expanded, there remains an overall lack of studies assessing the response of triploids to chemical treatments. It is essential that the response of triploids to disease treatments be characterised to validate their suitability for commercial production. This study aimed to investigate and compare the stress and immune responses of triploid and diploid Atlantic salmon following an experimental treatment with hydrogen peroxide (H2O2). A dose response test was first undertaken to determine a suitable test dose for both diploid and triploid Atlantic salmon. Following this, diploids and triploids were exposed to H2O2 (1800 ppm) for 20 min, as per commercial practices, after which blood glucose and lactate, and plasma cortisol and lysozyme were measured, along with the expression of oxidative stress and immune-related genes. In the first 6 h post-exposure to H2O2, comparable mortalities occurred in both diploid and triploid Atlantic salmon. Cortisol, glucose and lactate were not significantly influenced by ploidy suggesting that, physiologically, triploid Atlantic salmon are able to cope with the stress associated with H2O2 exposure as well as their diploid counterparts. Exposure to H2O2 significantly elevated the expression of cat and sod2 in diploid livers and gr, il1β and crp/sap1b in diploid gills, while it significantly decreased the expression of saa5 and crp/sap1a in diploid gills. In triploids, the expression levels of cat, hsp70, sod1, saa5, crp/sap1a and crp/sap1b in liver was significantly higher in fish exposed to H2O2 compared to control fish. The expression of gr, sod1 and il1β in triploid gills was also elevated in response to H2O2 exposure. This study represents the first experimental evidence of the effects of H2O2 exposure on triploid Atlantic salmon and continues to support their application into commercial production. This study represents the first testing of H2O2 in triploid Atlantic salmon. Following H2O2 exposure, triploid and diploid Atlantic salmon showed similar levels of cortisol, glucose and lactate. Ploidy differences in gene expression may be linked to extra genetic material in triploid cells, but requires further study.
登录
查看更多内容
影响因子：
2.4
作者：
Chalmers L;Taylor JF;Roy W;Preston AC;Migaud H;Adams A
通讯作者：
Adams A
影响因子：
2.5
作者：
Costello, Mark J.
通讯作者：
Costello, Mark J.
影响因子：
2.9
作者：
Bruno, D. W.;Raynard, R. S.
通讯作者：
Raynard, R. S.
影响因子：
4.7
作者：
Fast, M. D.;Johnson, S. C.;Jones, S. R. M.
通讯作者：
Jones, S. R. M.
影响因子：
2.5
作者：
Adams, M. B.;Crosbie, P. B. B.;Nowak, B. F.
通讯作者：
Nowak, B. F.