Identification of proteasome subunit beta type 6 (PSMB6) associated with deltamethrin resistance in mosquitoes by proteomic and bioassay analyses.
Identification of proteasome subunit beta type 6 (PSMB6) associated with deltamethrin resistance in mosquitoes by proteomic and bioassay analyses.
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通过蛋白质组学和生物测定分析鉴定与蚊子溴氰菊酯抗性相关的蛋白酶体亚基 6 型 (PSMB6)
DOI：
10.1371/journal.pone.0065859
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhu C
中科院分区：
文献类型：
--
作者：
Sun L;Ye Y;Sun H;Yu J;Zhang L;Sun Y;Zhang D;Ma L;Shen B;Zhu C
溴氰菊酯（DM）杀虫剂因其高效、低毒、对环境影响小等优点，目前在世界范围内得到推广。由于杀虫剂的广泛使用和不当使用而产生的抗药性已成为虫媒病害防治的主要障碍。抗性的形成是一个涉及多个基因的复杂的动态过程。为了更好地了解可能参与DM抗性的分子机制，采用蛋白质组学方法筛选DM敏感和抗性蚊子细胞中差异表达的蛋白质。通过双向电泳（2-DE）和质谱（MS）鉴定了27个差异表达蛋白。泛素-蛋白酶体系统的四个成员在DM抗性细胞中显著升高，表明泛素-蛋白酶体途径可能在DM抗性中起重要作用。蛋白酶体β亚基6（PSMB 6）是20 S蛋白酶体亚基家族的成员，是26 S蛋白酶体的核心蛋白。我们采用药物抑制剂和分子生物学方法研究了PSMB 6在DM耐药中的作用：使用蛋白酶体抑制剂MG-132和硼替佐米抑制蛋白酶体活性，设计siRNA阻断PSMB 6的功能。结果表明，MG-132和硼替佐米均增加了DM抗性细胞和抗性幼虫的敏感性。此外，PSMB 6敲低降低DM处理下的细胞活力。综上所述，我们的研究表明PSMB 6与蚊子中的DM抗性相关，并且蛋白酶体抑制剂如MG-132或硼替佐米适合用作媒介控制的DM抑制剂。
Deltamethrin (DM) insecticides are currently being promoted worldwide for mosquito control, because of the high efficacy, low mammalian toxicity and less environmental impact. Widespread and improper use of insecticides induced resistance, which has become a major obstacle for the insect-borne disease management. Resistance development is a complex and dynamic process involving many genes. To better understand the possible molecular mechanisms involved in DM resistance, a proteomic approach was employed for screening of differentially expressed proteins in DM-susceptible and -resistant mosquito cells. Twenty-seven differentially expressed proteins were identified by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Four members of the ubiquitin-proteasome system were significantly elevated in DM-resistant cells, suggesting that the ubiquitin-proteasome pathway may play an important role in DM resistance. Proteasome subunit beta type 6 (PSMB6) is a member of 20S proteasomal subunit family, which forms the proteolytic core of 26S proteasome. We used pharmaceutical inhibitor and molecular approaches to study the contributions of PSMB6 in DM resistance: the proteasome inhibitor MG-132 and bortezomib were used to suppress the proteasomal activity and siRNA was designed to block the function of PSMB6. The results revealed that both MG-132 and bortezomib increased the susceptibility in DM-resistant cells and resistance larvae. Moreover, PSMB6 knockdown decreased cellular viability under DM treatment. Taken together, our study indicated that PSMB6 is associated with DM resistance in mosquitoes and that proteasome inhibitors such as MG-132 or bortezomib are suitable for use as a DM synergist for vector control.
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影响因子：
5.4
作者：
Phuc HK;Andreasen MH;Burton RS;Vass C;Epton MJ;Pape G;Fu G;Condon KC;Scaife S;Donnelly CA;Coleman PG;White-Cooper H;Alphey L
通讯作者：
Alphey L
影响因子：
6.7
作者：
Rivero A;Vézilier J;Weill M;Read AF;Gandon S
通讯作者：
Gandon S
影响因子：
1.6
作者：
Mahande AM;Dusfour I;Matias JR;Kweka EJ
通讯作者：
Kweka EJ
DOI：
10.1073/pnas.1200854109
发表时间：
2012-04-17
影响因子：
11.1
作者：
Geng, Fuqiang;Tansey, William P.
通讯作者：
Tansey, William P.
影响因子：
4.4
作者：
Ma, Minyue;Guo, Xuejiang;Zhou, Qi
通讯作者：
Zhou, Qi