Bacterial diversity in two Neonatal Intensive Care Units (NICUs).
Bacterial diversity in two Neonatal Intensive Care Units (NICUs).
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DOI：
10.1371/journal.pone.0054703
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kelley ST
中科院分区：
文献类型：
--
作者：
Hewitt KM;Mannino FL;Gonzalez A;Chase JH;Caporaso JG;Knight R;Kelley ST
新生儿重症监护病房 (NICU) 中的婴儿特别容易受到机会性感染。受感染的婴儿死亡率很高，幸存者往往患有终生神经系统疾病。许多新生儿重症监护病房 (NICU) 感染的原因未得到诊断，而且无生命医院环境 (IHE) 在感染传播中的重要性存在争议。我们使用独立于培养物的下一代测序来调查圣地亚哥两个新生儿重症监护室的细菌多样性，并追踪这些环境中微生物的来源。从两个三级 NICU 设施中采集了 30 个 IHE 样本。我们从这些样本中提取 DNA，并使用“通用”条形码引物扩增细菌小亚基 (16S) 核糖体 RNA 基因序列。将纯化的 PCR 产物汇集到单个反应中进行焦磷酸测序，并使用 QIIME 分析数据。平均而言，我们在 NICU IHE 中的每个样本中检测到 93+/-39（平均+/-标准差）个细菌属。许多细菌属包括已知的机会病原体，并且许多与皮肤相关（例如丙酸杆菌）。在一间新生儿重症监护病房（NICU）中，我们还在高比例的表面样本中检测到了粪便大肠菌（肠杆菌目）。将这些源自 NICU 的序列与之前发表的其他室内环境（办公室、卫生间和医疗机构）以及人类和土壤相关环境的高通量 16S rRNA 扩增子研究进行比较，发现大多数 NICU 样本与典型的建筑物表面和空气样本相似，但以肠杆菌科细菌为主的 IHE 是一个明显的例外。我们的研究结果证明 NICU IHE 具有高度多样性的人类相关细菌，并证明了分子方法在识别和跟踪 NICU 细菌多样性方面的潜在效用。
Infants in Neonatal Intensive Care Units (NICUs) are particularly susceptible to opportunistic infection. Infected infants have high mortality rates, and survivors often suffer life-long neurological disorders. The causes of many NICU infections go undiagnosed, and there is debate as to the importance of inanimate hospital environments (IHEs) in the spread of infections. We used culture-independent next-generation sequencing to survey bacterial diversity in two San Diego NICUs and to track the sources of microbes in these environments. Thirty IHE samples were collected from two Level-Three NICU facilities. We extracted DNA from these samples and amplified the bacterial small subunit (16S) ribosomal RNA gene sequence using ‘universal’ barcoded primers. The purified PCR products were pooled into a single reaction for pyrosequencing, and the data were analyzed using QIIME. On average, we detected 93+/−39 (mean +/− standard deviation) bacterial genera per sample in NICU IHEs. Many of the bacterial genera included known opportunistic pathogens, and many were skin-associated (e.g., Propionibacterium). In one NICU, we also detected fecal coliform bacteria (Enterobacteriales) in a high proportion of the surface samples. Comparison of these NICU-derived sequences to previously published high-throughput 16S rRNA amplicon studies of other indoor environments (offices, restrooms and healthcare facilities), as well as human- and soil-associated environments, found the majority of the NICU samples to be similar to typical building surface and air samples, with the notable exception of the IHEs which were dominated by Enterobacteriaceae. Our findings provide evidence that NICU IHEs harbor a high diversity of human-associated bacteria and demonstrate the potential utility of molecular methods for identifying and tracking bacterial diversity in NICUs.
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影响因子：
3.7
作者：
Hewitt KM;Gerba CP;Maxwell SL;Kelley ST
通讯作者：
Kelley ST
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
4.4
作者：
Lauber, Christian L.;Hamady, Micah;Fierer, Noah
通讯作者：
Fierer, Noah
影响因子：
--
作者：
Martínez, JA;Ruthazer, R;Snydman, DR
通讯作者：
Snydman, DR