Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets.
Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets.
复制标题
表达树突状细胞靶向肽融合 PEDV COE 蛋白的重组干酪乳杆菌口服免疫 PEDV
DOI：
10.3390/v10030106
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发表时间：
2018-03-01
期刊：
影响因子：
--
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Hou X;Jiang X;Jiang Y;Tang L;Xu Y;Qiao X;Min L;Wen C;Ma G;Li Y
猪流行性腹泻（PED）是新生仔猪的一种高度传染性疾病。在我们之前的研究中，我们开发了一种表达树突状细胞（DC）靶向肽与猪流行性腹泻病毒（PEDV） COE抗原融合的干酪乳杆菌口服疫苗（pPG-COE-DCpep/L393）。该疫苗在小鼠中诱导了显著水平的抗PEDV特异性IgG和IgA抗体反应，表明了一种对抗PEDV感染的潜在策略。本研究采用pPG-COE-DCpep/L393进行新生仔猪PEDV口服疫苗接种。然后我们评估了pPG-COE-DCpep/L393的免疫反应和保护效果。间接酶联免疫吸附试验（ELISA）表明，重组乳酸菌疫苗可引起特异性全身和粘膜免疫应答。pPG-COE-DCpep/L393和PEDV感染介导的t辅助细胞表现为Th1表型。组织病理学结果表明，pPG-COE-DCpep/L393能促进仔猪淋巴细胞增殖，有效保护仔猪免受PEDV感染。转化生长因子-β水平表明重组乳杆菌疫苗在PEDV感染时肠系膜淋巴结的抗炎反应中起作用。这些结果表明pPG-COE-DCpep/L393是一种潜在的PEDV疫苗。
Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen was developed. This vaccine induced significant levels of anti-PEDV specific IgG and IgA antibody responses in mice, indicating a potential strategy against PEDV infection. In this study, pPG-COE-DCpep/L393 was used for oral vaccination of newborn piglets against PEDV. We then assessed the immune responses and protection efficacy of pPG-COE-DCpep/L393. An indirect enzyme-linked immunosorbent assay (ELISA) showed that the recombinant Lactobacillus vaccine elicits a specific systemic and mucosal immune response. The T-helper cells mediated by pPG-COE-DCpep/L393 and PEDV infection display a Th1 phenotype. The histopathological results showed that pPG-COE-DCpep/L393 promotes lymphocyte proliferation and effectively protects piglets against PEDV infection. The transforming growth factor-β level indicated that the recombinant Lactobacillus vaccine plays a role in anti-inflammatory responses in mesenteric lymph nodes during PEDV infection. These results show that pPG-COE-DCpep/L393 is a potential vaccine against PEDV infection.
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