Human occupancy as a source of indoor airborne bacteria.
Human occupancy as a source of indoor airborne bacteria.
复制标题
DOI：
10.1371/journal.pone.0034867
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Peccia J
中科院分区：
文献类型：
--
作者：
Hospodsky D;Qian J;Nazaroff WW;Yamamoto N;Bibby K;Rismani-Yazdi H;Peccia J
暴露于室内特定的空气传播细菌与感染性和非感染性不良健康结果有关。然而，悬浮在室内空气中的细菌的来源和起源并不清楚。这项研究提出了室内空气中细菌浓度升高的证据，由于人类居住，并调查这些细菌的来源。样本收集在大学教室，而占用和空置时。总颗粒质量浓度，细菌基因组浓度，和细菌系统发育的人口，其特征在于在室内，室外，通风管道供应空气，以及在通风系统过滤器的灰尘和地板灰尘。居住增加了室内空气PM10和PM2.5粒级中的气溶胶总质量和细菌基因组浓度，PM10中空气中细菌基因组浓度增加了近两个数量级。在单位质量的基础上，与空气中的颗粒相比，地板灰尘富含细菌基因组。室内空气中的细菌种群和潜在的来源之间的定量比较表明，再悬浮地板灰尘是一个重要的贡献者，在占用期间的细菌气溶胶种群。控制来自地板的再悬浮的实验表明，直接人类脱落也可能显着影响室内空气中颗粒物的浓度。在室内空气和地板灰尘中发现的人类皮肤、鼻孔和头发特有的高含量细菌表明，地板是人类相关细菌的重要储存库，脱落的皮肤细胞的直接颗粒脱落及其随后的再悬浮强烈影响了这种人类居住环境中空气传播的细菌种群结构。在公共室内环境中，可能会发生吸入暴露于其他当前或以前的人类居住者所散发的微生物。
Exposure to specific airborne bacteria indoors is linked to infectious and noninfectious adverse health outcomes. However, the sources and origins of bacteria suspended in indoor air are not well understood. This study presents evidence for elevated concentrations of indoor airborne bacteria due to human occupancy, and investigates the sources of these bacteria. Samples were collected in a university classroom while occupied and when vacant. The total particle mass concentration, bacterial genome concentration, and bacterial phylogenetic populations were characterized in indoor, outdoor, and ventilation duct supply air, as well as in the dust of ventilation system filters and in floor dust. Occupancy increased the total aerosol mass and bacterial genome concentration in indoor air PM10 and PM2.5 size fractions, with an increase of nearly two orders of magnitude in airborne bacterial genome concentration in PM10. On a per mass basis, floor dust was enriched in bacterial genomes compared to airborne particles. Quantitative comparisons between bacterial populations in indoor air and potential sources suggest that resuspended floor dust is an important contributor to bacterial aerosol populations during occupancy. Experiments that controlled for resuspension from the floor implies that direct human shedding may also significantly impact the concentration of indoor airborne particles. The high content of bacteria specific to the skin, nostrils, and hair of humans found in indoor air and in floor dust indicates that floors are an important reservoir of human-associated bacteria, and that the direct particle shedding of desquamated skin cells and their subsequent resuspension strongly influenced the airborne bacteria population structure in this human-occupied environment. Inhalation exposure to microbes shed by other current or previous human occupants may occur in communal indoor environments.
登录
查看更多内容
DOI：
10.1073/pnas.0608255104
发表时间：
2007-01-02
影响因子：
11.1
作者：
Brodie, Eoin L.;DeSantis, Todd Z.;Andersen, Gary L.
通讯作者：
Andersen, Gary L.
影响因子：
11.4
作者：
Ferro, AR;Kopperud, RJ;Hildemann, LM
通讯作者：
Hildemann, LM
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
--
作者：
Fox, A;Harley, W;Larsson, L
通讯作者：
Larsson, L
影响因子：
--
作者：
Fox, Karen;Fox, Alvin;Salzberg, Deborah
通讯作者：
Salzberg, Deborah