Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program.
Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program.
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DOI：
10.1371/journal.pone.0016066
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发表时间：
2011-01-12
期刊：
影响因子：
3.7
通讯作者：
Speybroeck N
中科院分区：
文献类型：
--
作者：
Yewhalaw D;Wassie F;Steurbaut W;Spanoghe P;Van Bortel W;Denis L;Tessema DA;Getachew Y;Coosemans M;Duchateau L;Speybroeck N
室内滞留喷洒、驱虫蚊帐和长效驱虫蚊帐是疟疾预防和控制战略的关键组成部分。然而，蚊子对建议用于室内滞留喷洒和/或经杀虫剂处理过的蚊帐/长效驱虫蚊帐的杀虫剂产生抗药性，将影响以杀虫剂为基础的疟疾病媒控制。我们评估了阿拉伯按蚊对疟疾控制中使用的杀虫剂的敏感性水平，表征了抗性的基本机制，并评估了公共卫生使用杀虫剂在抗性选择中的作用。安的敏感性状态。2009年8月至9月，使用世卫组织生物测定试验评估了埃塞俄比亚阿拉伯小蠊对滴滴涕、氯菊酯、溴氰菊酯、马拉硫磷和残杀威的毒性。分别采用AS-PCR和PCR-RFLP方法对藓类标本进行敲低抗性（kdr）和不敏感乙酰胆碱酯酶（ace-1 R）突变筛查。采用气相色谱-电子捕获检测器法测定了人类居住地土壤及周围环境中滴滴涕的残留量。一个.阿拉伯小蠊对DDT、氯菊酯、溴氰菊酯、马拉硫磷抗性，对残杀威敏感。在284例标本中发现了280例西非kdr等位基因，频率范围为95%至100%。在对等位基因评分的所有标本中均未检测到Ace-1 R突变。此外，在人类住所的土壤样本中发现了滴滴涕残留物，但在周围环境中没有发现。所观察到的多重抗性加上高kdr频率的发生在人口的An。阿拉伯疟原虫可能对埃塞俄比亚的疟疾病媒控制方案产生深远影响。这就迫切需要实施合理的抗药性管理战略和综合病媒控制干预措施。
Indoor Residual Spraying (IRS), insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) are key components in malaria prevention and control strategy. However, the development of resistance by mosquitoes to insecticides recommended for IRS and/or ITNs/LLINs would affect insecticide-based malaria vector control. We assessed the susceptibility levels of Anopheles arabiensis to insecticides used in malaria control, characterized basic mechanisms underlying resistance, and evaluated the role of public health use of insecticides in resistance selection. Susceptibility status of An. arabiensis was assessed using WHO bioassay tests to DDT, permethrin, deltamethrin, malathion and propoxur in Ethiopia from August to September 2009. Mosquito specimens were screened for knockdown resistance (kdr) and insensitive acetylcholinesterase (ace-1R) mutations using AS-PCR and PCR-RFLP, respectively. DDT residues level in soil from human dwellings and the surrounding environment were determined by Gas Chromatography with Electron Capture Detector. An. arabiensis was resistant to DDT, permethrin, deltamethrin and malathion, but susceptible to propoxur. The West African kdr allele was found in 280 specimens out of 284 with a frequency ranged from 95% to 100%. Ace-1R mutation was not detected in all specimens scored for the allele. Moreover, DDT residues were found in soil samples from human dwellings but not in the surrounding environment. The observed multiple-resistance coupled with the occurrence of high kdr frequency in populations of An. arabiensis could profoundly affect the malaria vector control programme in Ethiopia. This needs an urgent call for implementing rational resistance management strategies and integrated vector control intervention.
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影响因子：
3
作者：
Akogbeto, Martin C.;Padonou, Gil Germain;Yadouleton, Anges
通讯作者：
Yadouleton, Anges
影响因子：
2.1
作者：
Dasgupta, Ranjit;Free, Heather M.;Christensen, Bruce M.
通讯作者：
Christensen, Bruce M.
影响因子：
3
作者：
Bass, Chris;Nikou, Dimitra;Donnelly, Martin J.;Williamson, Martin S.;Ranson, Hilary;Ball, Amanda;Vontas, John;Field, Linda M.
通讯作者：
Field, Linda M.
DOI：
10.4269/ajtmh.1987.37.37
发表时间：
1987-07-01
影响因子：
3.3
作者：
COLLINS, FH;MENDEZ, MA;FINNERTY, V
通讯作者：
FINNERTY, V
DOI：
10.4269/ajtmh.2008.78.298
发表时间：
2008-02-01
影响因子：
3.3
作者：
Djogbenou, Luc;Dabire, Roch;Chandre, Fabrice
通讯作者：
Chandre, Fabrice