Identification of differentially expressed genes between sorghum genotypes with contrasting nitrogen stress tolerance by genome-wide transcriptional profiling.
Identification of differentially expressed genes between sorghum genotypes with contrasting nitrogen stress tolerance by genome-wide transcriptional profiling.
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DOI：
10.1186/1471-2164-15-179
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发表时间：
2014-03-05
期刊：
影响因子：
4.4
通讯作者：
Dweikat I
中科院分区：
文献类型：
--
作者：
Gelli M;Duo Y;Konda AR;Zhang C;Holding D;Dweikat I
高粱是一种重要的谷类作物，其需要大量的氮肥来实现商业产量。鉴定高粱耐低氮基因有助于了解高粱耐低氮的分子机制，也有助于通过分子标记辅助选择或基因转化进行高粱的遗传改良。在这项研究中，我们比较了7个高粱基因型根组织的转录组具有差异的低氮胁迫反应。利用Illumina RNA测序技术检测了4个耐低氮高粱基因型（三七散、China 17、KS 78和高氮素利用效率高粱）与3个敏感高粱基因型（CK 60、BTx 623和低氮素利用效率高粱）之间的差异表达基因（DEG）。在敏感的基因型，N-压力增加了丰富的DEG转录与应激反应，包括氧化应激和刺激是丰富的。耐氮基因型通过产生更大的根量来有效吸收养分，从而适应氮素缺乏。在耐受基因型中，与高亲和力硝酸盐转运蛋白（NRT 2.2，NRT 2.3，NRT 2.5和NRT 2.6）和赖氨酸组氨酸转运蛋白1（LHT 1）相关的转录本丰度较高，可能表明无机和有机形式氮的吸收效率提高。在耐氮基因型中，较高丰度的SEC 14胞质因子家族蛋白转录本可导致膜稳定性和对氮胁迫的耐受性增加。N-胁迫耐受和敏感基因型之间的转录组比较揭示了几个共同的DEG成绩单。通过比较在全氮条件下生长的基因型的转录组，进一步评估了其中一些DEG。DEG转录本在耐氮基因型中表达量较高，可用于转基因高粱及相关作物的敏感基因型中过量表达，以提高其对氮胁迫的耐受性，从而提高氮素利用效率，促进农业可持续发展。
Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having differential response to low-N stress. Illumina RNA-sequencing detected several common differentially expressed genes (DEGs) between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three sensitive genotypes (CK60, BTx623 and low-NUE bulk). In sensitive genotypes, N-stress increased the abundance of DEG transcripts associated with stress responses including oxidative stress and stimuli were abundant. The tolerant genotypes adapt to N deficiency by producing greater root mass for efficient uptake of nutrients. In tolerant genotypes, higher abundance of transcripts related to high affinity nitrate transporters (NRT2.2, NRT2.3, NRT2.5, and NRT2.6) and lysine histidine transporter 1 (LHT1), may suggest an improved uptake efficiency of inorganic and organic forms of nitrogen. Higher abundance of SEC14 cytosolic factor family protein transcript in tolerant genotypes could lead to increased membrane stability and tolerance to N-stress. Comparison of transcriptomes between N-stress tolerant and sensitive genotypes revealed several common DEG transcripts. Some of these DEGs were evaluated further by comparing the transcriptomes of genotypes grown under full N. The DEG transcripts showed higher expression in tolerant genotypes could be used for transgenic over-expression in sensitive genotypes of sorghum and related crops for increased tolerance to N-stress, which results in increased nitrogen use efficiency for sustainable agriculture.
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影响因子：
2.9
作者：
Cai, Hongmei;Lu, Yongen;Lian, Xingming
通讯作者：
Lian, Xingming
影响因子：
4.4
作者：
Hao QN;Zhou XA;Sha AH;Wang C;Zhou R;Chen SL
通讯作者：
Chen SL
影响因子：
7.3
作者：
Bi, Yong-Mei;Kant, Surya;Rothstein, Steven J.
通讯作者：
Rothstein, Steven J.
影响因子：
56.9
作者：
Guo, J. H.;Liu, X. J.;Zhang, F. S.
通讯作者：
Zhang, F. S.
DOI：
10.1073/pnas.83.21.8073
发表时间：
1986-11-01
影响因子：
11.1
作者：
CRAWFORD, NM;CAMPBELL, WH;DAVIS, RW
通讯作者：
DAVIS, RW