The relationship between transgenerational acquired resistance and global DNA methylation in Arabidopsis.
The relationship between transgenerational acquired resistance and global DNA methylation in Arabidopsis.
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DOI：
10.1038/s41598-018-32448-5
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发表时间：
2018-10-03
影响因子：
4.6
通讯作者：
Ton J
中科院分区：
文献类型：
--
作者：
Stassen JHM;López A;Jain R;Pascual-Pardo D;Luna E;Smith LM;Ton J
重病植物的后代产生跨代获得性抗性（TAR）。在拟南芥中，TAR可以在无胁迫的一代中传播。尽管DNA甲基化与TAR的调控有关，但TAR与整体DNA甲基化之间的关系尚不清楚。在这里，我们描述了疾病暴露后不同世代表达tar的拟南芥的甲基组。胞嘧啶甲基化的全球聚类揭示了F3代的tar相关模式，而F1代则没有。在基因体中，大多数差异甲基化位点（dmp）发生在CG背景下。患病1代后，无应激2代后，F3后代的TAR低于患病3代后F3后代的TAR。这种TAR有效性的差异与胞嘧啶亚群位置的差异甲基化强度成正比。将突变积累系中tar相关的dmp与先前表征的胞嘧啶甲基化进行比较，发现祖先疾病胁迫优先作用于甲基化不稳定的胞嘧啶位置，但也扩展到甲基化稳定的位置。因此，祖先疾病的tar相关影响超出了DNA甲基化的随机变异。我们的研究表明，拟南芥表观基因组对前几代的疾病有全局反应，我们讨论了它对TAR的贡献。
Progeny of heavily diseased plants develop transgenerational acquired resistance (TAR). In Arabidopsis, TAR can be transmitted over one stress-free generation. Although DNA methylation has been implicated in the regulation of TAR, the relationship between TAR and global DNA methylation remains unknown. Here, we characterised the methylome of TAR-expressing Arabidopsis at different generations after disease exposure. Global clustering of cytosine methylation revealed TAR-related patterns in the F3 generation, but not in the F1 generation. The majority of differentially methylated positions (DMPs) occurred at CG context in gene bodies. TAR in F3 progeny after one initial generation of disease, followed by two stress-free generations, was lower than TAR in F3 progeny after three successive generations of disease. This difference in TAR effectiveness was proportional to the intensity of differential methylation at a sub-set of cytosine positions. Comparison of TAR-related DMPs with previously characterised cytosine methylation in mutation accumulation lines revealed that ancestral disease stress preferentially acts on methylation-labile cytosine positions, but also extends to methylation-stable positions. Thus, the TAR-related impact of ancestral disease extends beyond stochastic variation in DNA methylation. Our study has shown that the Arabidopsis epigenome responds globally to disease in previous generations and we discuss its contribution to TAR.
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DOI：
10.1038/nrm4043
发表时间：
2015-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Du J;Johnson LM;Jacobsen SE;Patel DJ
通讯作者：
Patel DJ
影响因子：
14.9
作者：
Feng H;Conneely KN;Wu H
通讯作者：
Wu H
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
从头响应的拟南芥突变和表述的环境响应式全基因组的积累。
影响因子：
7
作者：
Jiang C;Mithani A;Belfield EJ;Mott R;Hurst LD;Harberd NP
通讯作者：
Harberd NP
影响因子：
64.5
作者：
Kawakatsu T;Huang SC;Jupe F;Sasaki E;Schmitz RJ;Urich MA;Castanon R;Nery JR;Barragan C;He Y;Chen H;Dubin M;Lee CR;Wang C;Bemm F;Becker C;O'Neil R;O'Malley RC;Quarless DX;1001 Genomes Consortium;Schork NJ;Weigel D;Nordborg M;Ecker JR
通讯作者：
Ecker JR