Comparative tissue proteomics reveals unique action mechanisms of vaccine adjuvants.
Comparative tissue proteomics reveals unique action mechanisms of vaccine adjuvants.
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DOI：
10.1016/j.isci.2022.105800
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发表时间：
2023-01-20
期刊：
影响因子：
5.8
通讯作者：
Chen, Xinyuan
中科院分区：
文献类型：
--
作者：
Li, Yibo;Li, Zhuofan;Chen, Xinyuan
最近开发了射频佐剂（RFA）来加强流感疫苗接种，而没有化学佐剂因其物理性质而产生的安全问题。然而，RFA的作用机制在很大程度上仍然未知。组学技术为确定RFA的分子机制提供了新的机会。本研究利用比较组织蛋白质组学来探索物理RFA的分子机制。比较RFA和化学佐剂（Alum、AddaVax、MPL、MPL/Alum）诱导的组织蛋白质组变化，鉴定出14种RFA专门诱导的蛋白质，其中热休克蛋白（HSP）70由于其已知的免疫调节功能而被选择用于进一步分析。RFA在HSP 70敲除小鼠中增强卵清蛋白和大流行性流感疫苗接种的能力比野生型小鼠弱得多，暗示了HSP 70在RFA效应中的关键作用。本研究支持比较组织蛋白质组学是研究疫苗佐剂分子机制的有效工具。比较组织蛋白质组学鉴定了佐剂独特诱导的蛋白质MF 59模拟AddaVax佐剂显示对局部组织蛋白质组的影响最大Radiofrequency佐剂显示对局部组织蛋白质组的影响最小HSP 70被鉴定为射频佐剂效应的关键参与者免疫;医学生物技术;蛋白质组学
Radiofrequency adjuvant (RFA) was recently developed to boost influenza vaccination without the safety concerns of chemical adjuvants due to their physical nature. Yet, the action mechanisms of RFA remain largely unknown. Omics techniques offer new opportunities to identify molecular mechanisms of RFA. This study utilized comparative tissue proteomics to explore molecular mechanisms of the physical RFA. Comparison of RFA and chemical adjuvant (Alum, AddaVax, MPL, MPL/Alum)-induced tissue proteome changes identified 14 exclusively induced proteins by RFA, among which heat shock protein (HSP) 70 was selected for further analysis due to its known immune-modulating functions. RFA showed much weakened ability to boost ovalbumin and pandemic influenza vaccination in HSP70 knockout than wild-type mice, hinting crucial roles of HSP70 in RFA effects. This study supports comparative tissue proteomics to be an effective tool to study molecular mechanisms of vaccine adjuvants. Comparative tissue proteomics identified uniquely induced proteins by adjuvants MF59-mimetic AddaVax adjuvant showed the most impact on local tissue proteome Radiofrequency adjuvant showed the least impact on local tissue proteome HSP70 was identified as a key player in radiofrequency adjuvant effects Immunity; Medical biotechnology; Proteomics
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影响因子：
7.3
作者：
Awate S;Babiuk LA;Mutwiri G
通讯作者：
Mutwiri G
DOI：
10.1096/fj.202101703r
发表时间：
2022-03
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Li Z;Kim KH;Bhatnagar N;Park BR;Jeeva S;Jung YJ;Raha J;Kang SM;Chen X
通讯作者：
Chen X
影响因子：
7.8
作者：
Di Pasquale A;Preiss S;Tavares Da Silva F;Garçon N
通讯作者：
Garçon N
影响因子：
7.3
作者：
Burny, Wivine;Callegaro, Andrea;Didierlaurent, Arnaud M.
通讯作者：
Didierlaurent, Arnaud M.
影响因子：
5.6
作者：
Elhodaky M;Diamond AM
通讯作者：
Diamond AM