Collection and detection of SARS-CoV-2 in exhaled breath using face mask.
Collection and detection of SARS-CoV-2 in exhaled breath using face mask.
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DOI：
10.1371/journal.pone.0270765
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Shin, Sehyun
中科院分区：
文献类型：
--
作者：
Kim, Hwang-Soo;Lee, Hansol;Park, Junsoo;Abbas, Naseem;Kang, Seonghui;Hyun, Hakjun;Seong, Hye;Yoon, Jin Gu;Noh, Ji Yun;Kim, Woo Joo;Shin, Sehyun
口罩用于保护佩戴者免受有害外部空气的影响，并防止潜在感染佩戴者呼出的空气中的病毒传播给周围的人。在这项研究中，我们研究了口罩收集呼出气体中所含病毒的潜在用途，并通过各种分子测试检测了收集到的病毒。使用KF94口罩，选择内部静电过滤器进行病毒收集，并制定了口罩RNA提取方案。使用 PCR 和滚环扩增 (RCA) 测试以及四种不同的目标基因（N、E、RdRp 和 ORF1ab 基因）对佩戴的口罩样本中的病毒进行检测。本研究证实，口罩样本检测显示 SARS-CoV-2 结果呈阳性，类似于使用鼻咽拭子样本进行的 PCR 检测。此外，口罩中采集的核酸量随着佩戴时间的延长呈线性增加。这些结果表明，只需提交受试者佩戴的口罩，即可以无创、快速、简便的方式收集用于SARS-CoV-2检测的样本，这可以显着减少在机场或公共场所等待的麻烦以及对交叉感染的担忧。此外，预计小型化技术将在不久的将来将PCR检测整合到口罩上，基于口罩的自我诊断将在解决疫情方面发挥重要作用。
Face masks are used to protect the wearer from harmful external air and to prevent transmission of viruses from air exhaled by potentially infected wearers to the surrounding people. In this study, we examined the potential utility of masks for collecting viruses contained in exhaled breath and detected the collected viruses via various molecular tests. Using KF94 masks, the inner electrostatic filter was selected for virus collection, and an RNA extraction protocol was developed for the face mask. Virus detection in worn mask samples was performed using PCR and rolling circle amplification (RCA) tests and four different target genes (N, E, RdRp, and ORF1ab genes). The present study confirmed that the mask sample tests showed positive SARS-CoV-2 results, similar to the PCR tests using nasopharyngeal swab samples. In addition, the quantity of nucleic acid collected in the masks linearly increased with wearing time. These results suggest that samples for SARS-CoV-2 tests can be collected in a noninvasive, quick, and easy method by simply submitting worn masks from subjects, which can significantly reduce the hassle of waiting at airports or public places and concerns about cross-infection. In addition, it is expected that miniaturization technology will integrate PCR assays on face masks in the near future, and mask-based self-diagnosis would play a significant role in resolving the pandemic situation.
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影响因子：
12.6
作者：
Na W;Nam D;Lee H;Shin S
通讯作者：
Shin S
影响因子：
12.6
作者：
Kim HS;Abbas N;Shin S
通讯作者：
Shin S
影响因子：
5
作者：
Li R;Zhang M;Wu Y;Tang P;Sun G;Wang L;Mandal S;Wang L;Lang J;Passalacqua A;Subramaniam S;Song G
通讯作者：
Song G
DOI：
10.1038/s41579-021-00535-6
发表时间：
2021-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Leung NHL
通讯作者：
Leung NHL
DOI：
10.1016/j.jinf.2021.03.018
发表时间：
2021-06
期刊：
The Journal of infection
影响因子：
--
作者：
Williams CM;Pan D;Decker J;Wisniewska A;Fletcher E;Sze S;Assadi S;Haigh R;Abdulwhhab M;Bird P;Holmes CW;Al-Taie A;Saleem B;Pan J;Garton NJ;Pareek M;Barer MR
通讯作者：
Barer MR