Taking U out, with two nucleases?
Taking U out, with two nucleases?
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DOI：
10.1186/1471-2105-7-305
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发表时间：
2006-06-16
影响因子：
3
通讯作者：
Salavati R
中科院分区：
文献类型：
--
作者：
Mian IS;Worthey EA;Salavati R
REX 1和REX 2是RNA编辑复合物（编辑体）的蛋白质组分，并作为外切尿苷酰化酶发挥作用。REX 1和REX 2在编辑体中的确切作用尚不清楚，两种相关蛋白质存在的后果也未完全了解。在这里，进行了各种计算研究，以加强对锥虫和利什曼原虫属物种中雷克斯蛋白的结构和功能的理解。REX 1和REX 2的C-末端的核酸内切酶/核酸外切酶/磷酸酶（EEP）结构域的序列分析和同源性建模突出了所有EEP结构域共享的共同活性位点。系统发育分析表明，雷克斯蛋白包含一个独特的亚家族EEP结构域。布氏锥虫REX 1和REX 2以及利什曼原虫REX 1中EEP结构域的三维模型的检查表明先前表征的关键残基的变化可能在催化和确定底物特异性中是重要的。我们已经确定了雷克斯EEP结构域的特征，将其与其他家族成员区分开来，因此具有催化和底物结合的亚家族特异性决定因素。这些结果为雷克斯蛋白在RNA编辑中的作用的实验研究提供了具体的指导。
REX1 and REX2 are protein components of the RNA editing complex (the editosome) and function as exouridylylases. The exact roles of REX1 and REX2 in the editosome are unclear and the consequences of the presence of two related proteins are not fully understood. Here, a variety of computational studies were performed to enhance understanding of the structure and function of REX proteins in Trypanosoma and Leishmania species. Sequence analysis and homology modeling of the Endonuclease/Exonuclease/Phosphatase (EEP) domain at the C-terminus of REX1 and REX2 highlights a common active site shared by all EEP domains. Phylogenetic analysis indicates that REX proteins contain a distinct subfamily of EEP domains. Inspection of three-dimensional models of the EEP domain in Trypanosoma brucei REX1 and REX2, and Leishmania major REX1 suggests variations of previously characterized key residues likely to be important in catalysis and determining substrate specificity. We have identified features of the REX EEP domain that distinguish it from other family members and hence subfamily specific determinants of catalysis and substrate binding. The results provide specific guidance for experimental investigations about the role(s) of REX proteins in RNA editing.
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影响因子：
3
作者：
Mian IS;Worthey EA;Salavati R
通讯作者：
Salavati R
影响因子：
64.5
作者：
Aphasizhev, R;Sbicego, S;Simpson, L
通讯作者：
Simpson, L
影响因子：
16
作者：
Ernst, NL;Panicucci, B;Stuart, K
通讯作者：
Stuart, K
影响因子：
14.9
作者：
Dalgaard, JZ;Klar, AJ;Mian, IS
通讯作者：
Mian, IS
影响因子：
5.3
作者：
Law, JA;Huang, CE;Sollner-Webb, B
通讯作者：
Sollner-Webb, B