Modularity of select riboswitch expression platforms enables facile engineering of novel genetic regulatory devices.
Modularity of select riboswitch expression platforms enables facile engineering of novel genetic regulatory devices.
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DOI：
10.1021/sb4000096
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发表时间：
2013-08-16
影响因子：
4.7
通讯作者：
Batey, Robert T.
中科院分区：
文献类型：
--
作者：
Ceres, Pablo;Garst, Andrew D.;Marcano-Velazquez, Joan G.;Batey, Robert T.
基于RNA的生物传感器和调控装置因其在广泛的合成生物学应用中的潜力而受到极大的关注。工程化这些分子的主要困难之一是缺乏将感觉模块或适体连接到读出结构域的简便方法。这些努力通常需要广泛筛选或选择促进结构域间通信的序列。细菌已经进化出一种广泛存在的基因调控形式，称为核糖开关，它以足够的保真度执行这一任务，以控制正常细胞稳态所必需的生物合成和转运蛋白的表达。在这项工作中，我们证明了选择核糖开关读出域，称为表达平台，是模块化的，因为它们可以宿主各种天然和合成的适体，以创建新的嵌合RNA，在体外和体内调节转录。重要的是，该技术不需要选择将配体结合传递到调节结构域所需的装置特异性“通信模块”，从而能够快速工程化新的功能性RNA。
RNA-based biosensors and regulatory devices have received significant attention for their potential in a broad array of synthetic biology applications. One of the primary difficulties in engineering these molecules is the lack of facile methods to link sensory modules, or aptamers, to readout domains. Such efforts typically require extensive screening or selection of sequences that facilitate interdomain communication. Bacteria have evolved a widespread form of gene regulation known as riboswitches that perform this task with sufficient fidelity to control expression of biosynthetic and transport proteins essential for normal cellular homeostasis. In this work, we demonstrate that select riboswitch readout domains, called expression platforms, are modular in that they can host a variety of natural and synthetic aptamers to create novel chimeric RNAs that regulate transcription both in vitro and in vivo. Importantly, this technique does not require selection of device-specific “communication modules” required to transmit ligand binding to the regulatory domain, enabling rapid engineering of novel functional RNAs.
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影响因子：
14.9
作者：
Lee, JF;Hesselberth, JR;Ellington, AD
通讯作者：
Ellington, AD
影响因子：
14.9
作者：
Blouin, Simon;Chinnappan, Raja;Lafontaine, Daniel A.
通讯作者：
Lafontaine, Daniel A.
影响因子：
4.5
作者：
Lemay JF;Desnoyers G;Blouin S;Heppell B;Bastet L;St-Pierre P;Massé E;Lafontaine DA
通讯作者：
Lafontaine DA
影响因子：
6.1
作者：
Batey, Robert T.
通讯作者：
Batey, Robert T.
影响因子：
8.4
作者：
Michener, Joshua K.;Smolke, Christina D.
通讯作者：
Smolke, Christina D.