Identification of a Quorum Sensing System Regulating Capsule Polysaccharide Production and Biofilm Formation in Streptococcus zooepidemicus
Identification of a Quorum Sensing System Regulating Capsule Polysaccharide Production and Biofilm Formation in Streptococcus zooepidemicus
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调节兽疫链球菌荚膜多糖产生和生物膜形成的群体感应系统的鉴定
DOI：
10.3389/fcimb.2019.00121
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发表时间：
2019-04
影响因子：
5.7
通讯作者：
Liu Hao
中科院分区：
文献类型：
--
作者：
Xie Zhoujie;Meng Kai;Yang Xiaoli;Liu Jie;Yu Jie;Zheng Chunyang;Cao Wei;Liu Hao
兽疫链球菌是包括人类在内的多种物种的重要条件致病菌。该菌也是工业生产透明质酸(HA)的主要生产菌，透明质酸是其胶囊多糖的成分。它的毒力和胶囊多糖的产生是如何被调控的，目前还知之甚少。细菌之间的细胞间化学信号为微生物群落提供了协调基因表达的机会，以促进群体行为，如致病性、胶囊多糖的产生等。然而，在动物疫链球菌中尚未阐明保守的细胞间信号系统。兽疫链球菌基因组中编码一个rgG调节基因(RgG)，与化脓性链球菌的rgg2和rgg3同源性较低。在RGG附近发现了一个编码一种新的短疏水肽(SHP)的小ORF(命名为shp)。我们发现，信息素的活性形式是短而疏水的(LLLLKLA)，对应于前肽SHP的C端7个氨基酸，显示出与链球菌中已报道的所有多肽信息素不同的序列。作为对SHP活性的响应，RGG作为转录激活剂，诱导shp的表达，形成正反馈回路。当RGG-shp途径失活时，细菌的社会行为，如胶囊多糖的产生和生物膜的形成，会受到显著的影响。这些数据首次证明RGG/SHP信号通路在兽疫链霉菌中包含一个活跃的群体感应系统。
Streptococcus zooepidemicus is an important opportunistic pathogen of several species including humans. This organism is also well-known as the main producing strain in industrial production of hyaluronic acid (HA), which is the component of its capsule polysaccharide. How its virulence and capsule polysaccharide production is regulated remains poorly understood. Intercellular chemical signaling among bacteria provides communities of microbes the opportunity to coordinate gene expression to facilitate group behavior, such as pathogenicity, capsule polysaccharide production, etc. Yet no conserved cell-to-cell signaling system has been elucidated in S. zooepidemicus. Encoded within the genome of S. zooepidemicus is one Rgg regulator encoding gene (rgg) with low similarity to both rgg2 and rgg3 from Streptococcus pyogenes. A small ORF (named as shp) encoding a novel short hydrophobic peptide (SHP) was found in the vicinity of rgg. We found that the active form of pheromone is short and hydrophobic (LLLLKLA), corresponding to the C terminal 7 amino acids of the pre-peptide Shp, which shows divergent sequence to all peptide pheromones reported in streptococci. In response to active SHP, Rgg functions as a transcriptional activator to induce the expression of shp, forming a positive feedback circuit. Bacteria social behaviors, such as capsule polysaccharide production and biofilm formation, were significantly affected when the rgg-shp pathway was inactivated. These data provide the first demonstration that Rgg/Shp signaling pathway comprises an active quorum sensing system in S. zooepidemicus.
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DOI：
10.3390/molecules14072535
发表时间：
2009-07-13
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Vu B;Chen M;Crawford RJ;Ivanova EP
通讯作者：
Ivanova EP
影响因子：
6.4
作者：
Liu L;Liu Y;Li J;Du G;Chen J
通讯作者：
Chen J
影响因子：
4.8
作者：
Junges R;Salvadori G;Shekhar S;Åmdal HA;Periselneris JN;Chen T;Brown JS;Petersen FC
通讯作者：
Petersen FC
DOI：
10.1007/978-4-431-54607-8
发表时间：
2014
期刊：
--
影响因子：
--
作者：
Hideharu Anazawa;S. Shimizu
通讯作者：
Hideharu Anazawa;S. Shimizu
影响因子：
3.1
作者：
VANDERIJN, I;KESSLER, RE
通讯作者：
KESSLER, RE