The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development.
The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development.
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ETS转录因子SPI-B是人浆细胞类动物树突状细胞发育所必需的。
DOI：
10.1084/jem.20041231
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发表时间：
2004-12-06
影响因子：
15.3
通讯作者：
Blom, B
中科院分区：
文献类型：
--
作者：
Schotte, R;Nagasawa, M;Weijer, K;Spits, H;Blom, B
已经确定了一些转录因子，它们充当了决定造血谱系决定的分子开关。我们最近报道了ETS转录因子SPI-B仅在浆细胞样树突状细胞(PDC)中表达，但不在髓系树突状细胞中表达。为了评估SPI-B是否是PDC发育所必需的，我们使用RNA干扰敲除方法特异性地沉默CD34+前体细胞中SPI-B蛋白的合成。我们观察到，在体外实验和体内注射重组激活基因2−/−γ普通−/−小鼠后，Spi-bmRNA的敲除都强烈地抑制了CD34+前体细胞向pDC的发育能力。观察到的影响仅限于PDC谱系，因为SPI-B基因敲除并未抑制前B细胞和CD14+髓系细胞的分化，但略有提高。相关ETS因子PU.1的敲除也抑制了CD34+细胞在体外向pDCs的发育。然而，与SPI-B相反，PU.1基因敲除也抑制了B细胞和髓细胞的发育。这些结果证实Spi-B是人类PDC发育的关键调节因子。
A number of transcription factors that act as molecular switches for hematopoietic lineage decisions have been identified. We recently described the ETS transcription factor Spi-B to be exclusively expressed in plasmacytoid dendritic cells (pDCs), but not in myeloid DCs. To assess whether Spi-B is required for pDC development we used an RNA interference knock down approach to specifically silence Spi-B protein synthesis in CD34+ precursor cells. We observed that a knock down of Spi-B mRNA strongly inhibited the ability of CD34+ precursor cells to develop into pDCs in both in vitro assays as well as in vivo upon injection into recombination activating gene 2−/− γ common−/− mice. The observed effects were restricted to the pDC lineage as the differentiation of pro–B cells and CD14+ myeloid cells was not inhibited but slightly elevated by Spi-B knock down. Knock down of the related ETS factor PU.1 also inhibited in vitro development of CD34+ cells into pDCs. However, in contrast to Spi-B, PU.1 knock down inhibited B cell and myeloid cell development as well. These results identify Spi-B as a key regulator of human pDC development.
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DOI：
10.1084/jem.20030107
发表时间：
2003-07-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
D'Amico A;Wu L
通讯作者：
Wu L
影响因子：
56.9
作者：
SCOTT, EW;SIMON, MC;SINGH, H
通讯作者：
SINGH, H
影响因子：
11.4
作者：
Su, GH;Chen, HM;Simon, MC
通讯作者：
Simon, MC
影响因子：
32.4
作者：
Wu, L;D'Amico, A;Shortman, K
通讯作者：
Shortman, K
DOI：
10.1084/jem.185.6.1101
发表时间：
1997-03-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Grouard G;Rissoan MC;Filgueira L;Durand I;Banchereau J;Liu YJ
通讯作者：
Liu YJ