Molecular epidemiology and temporal evolution of norovirus associated with acute gastroenteritis in Amazonas state, Brazil.
Molecular epidemiology and temporal evolution of norovirus associated with acute gastroenteritis in Amazonas state, Brazil.
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DOI：
10.1186/s12879-018-3068-y
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发表时间：
2018-04-02
影响因子：
3.7
通讯作者：
Gabbay YB
中科院分区：
文献类型：
--
作者：
Hernandez JM;Silva LD;Junior ECS;Bandeira RS;Rodrigues EAM;Lucena MSS;Costa STP;Gabbay YB
在全球范围内，诺如病毒（NoV）被认为是所有年龄段腹泻发作的最常见原因。尽管 GII.4 菌株具有广泛的遗传多样性，但它是最主要的菌株，并且与世界范围内的流行病有关。在这项研究中，我们对五年期间（2010-2014 年）NoV 阳性儿童的散发性腹泻病例进行了特征分析。对通过酶免疫分析 (EIA) 鉴定的总共 250 个 NoV 阳性样本进行了 RT-PCR 以及聚合酶和衣壳基因的部分核苷酸测序。使用二元分类进行系统发育分析以鉴定 NoV 基因型。此外，使用 BEAST 包中实施的 MCMC 方法，通过进化分析对 GII-4 变体的 P2 子域（衣壳）基因的序列进行了表征。使用蛋白质建模构建 3D 结构。系统发育分析表明，基因型 GII.4 (52.4% - 99/189)、变体 New Orleans_2009 和 Sydney_2012 占主导地位，其次是 GII.P7/GII.6，占 6.3% (12/189)。 GII.4 菌株的氨基酸分析显示了几个重要的氨基酸变化。发现了更高的进化率，悉尼变体为 7.7 × 10− 3，新奥尔良变体为 6.3 × 10− 3。根据进化分析，计算出最近共同祖先的时间（TMRCA）作为种群分化时间的估计。因此，新奥尔良和悉尼变体的 TMRCA 分别为 2008.7 和 2010.7。此外，我们还观察到新奥尔良和悉尼之间的过渡沿袭。这项研究描述了五年期间从巴西亚马逊州分离出的诺如病毒的不同毒株。考虑到NoV能够快速改变其抗原表位，因此通过流行病学研究持续监测NoV在社区中的发生和变化非常重要。这些结果有助于了解巴西亚马逊州的 NoV 分子流行病学及其进化动态。本文的在线版本 (10.1186/s12879-018-3068-y) 包含补充材料，可供授权用户使用。
Globally, Norovirus (NoV) is considered the most common cause of diarrheal episodes across all age groups. Despite its wide genetic diversity, the GII.4 strain is the most predominant and has been associated with epidemics worldwide. In this study, we characterized sporadic cases of diarrhea from NoV-positive children, during a five-year period (2010–2014). A total of 250 NoV-positive samples identified by an enzyme immunoassay (EIA) were subjected to RT-PCR and partial nucleotide sequencing for polymerase and capsid genes. Phylogenetic analysis was performed to identify NoV genotypes using the binary classification. In addition, sequences from the P2 subdomain (capsid) gene of GII-4 variants were characterized by evolutionary analyses, using the MCMC method implemented in the BEAST package. A 3D structure was built using protein modeling. Phylogenetic analysis demonstrated a predominance of genotype GII.4 (52.4% - 99/189), variants New Orleans_2009 and Sydney_2012 followed by GII.P7/GII.6 with 6.3% (12/189). Amino acid analyses of the GII.4 strains showed several important amino acid changes. A higher evolutionary rate was found, 7.7 × 10− 3 in the Sydney variant and 6.3 × 10− 3 in the New Orleans. Based in evolutionary analysis the time to the most recent common ancestor (TMRCA) has been calculated as estimates of the population divergence time. Thus, TMRCA for New Orleans and Sydney variant were 2008.7 and 2010.7, respectively. Also, we observed a lineage of transition between New Orleans and Sydney. This study describes the different strains of norovirus isolated from Amazonas state in Brazil during a five-year period. Considering that NoV are capable of changing their antigenic epitopes rapidly, a continuous surveillance is important to monitor the occurrence and changes of the NoV in the community through epidemiological studies. These results contribute to the understanding of NoV molecular epidemiology and its evolutionary dynamics in Amazonas state, Brazil. The online version of this article (10.1186/s12879-018-3068-y) contains supplementary material, which is available to authorized users.
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统一的诺如病毒术语和基因分型的提案。
影响因子：
2.7
作者：
Kroneman A;Vega E;Vennema H;Vinjé J;White PA;Hansman G;Green K;Martella V;Katayama K;Koopmans M
通讯作者：
Koopmans M
影响因子：
4.8
作者：
Mans J;Murray TY;Taylor MB
通讯作者：
Taylor MB
影响因子：
10.7
作者：
Minh BQ;Nguyen MA;von Haeseler A
通讯作者：
von Haeseler A
影响因子：
3.7
作者：
Fioretti, Julia Monassa;Bello, Gonzalo;Miagostovich, Marize Pereira
通讯作者：
Miagostovich, Marize Pereira
影响因子：
56.9
作者：
HOLLAND, J;SPINDLER, K;VANDEPOL, S
通讯作者：
VANDEPOL, S