The labial gene is required to terminate proliferation of identified neuroblasts in postembryonic development of the Drosophila brain.
The labial gene is required to terminate proliferation of identified neuroblasts in postembryonic development of the Drosophila brain.
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DOI：
10.1242/bio.20121966
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发表时间：
2012-10-15
期刊：
影响因子：
2.4
通讯作者：
Reichert H
中科院分区：
文献类型：
--
作者：
Kuert PA;Bello BC;Reichert H
果蝇正在发育的大脑已成为研究分子遗传机制的有用模型，这些分子遗传机制产生了复杂的神经元阵列，而这些神经元阵列是其他动物（包括哺乳动物）高级大脑的特征。果蝇的大脑发育在胚胎发生期间开始，并在随后的胚胎后阶段继续。在胚胎发生过程中，Hox 基因唇部在发育中的三头脑中表达，并且唇部功能丧失已被证明与大脑该部分区域神经元身份的丧失和严重的模式缺陷有关。然而，在大脑发育的胚胎后阶段（成人大脑中的大多数神经元是在这个阶段产生的），唇形基因或任何其他 Hox 基因的表达和功能尚不清楚。在这里，我们报告了果蝇胚胎后大脑发育过程中 Hox 基因作用的首次分析。我们发现，唇部最初在六个幼虫大脑神经母细胞中表达，其中只有四个产生晚期幼虫大脑中存在的唇部表达神经母细胞谱系。尽管这四种神经母细胞谱系中基于 MARCM 的阴唇克隆突变不会导致明显的表型，但在胚胎后大脑发育过程中确实会发生克隆阴唇功能丧失的惊人且意想不到的效果，即形成野生型大脑中不存在的两个异位神经母细胞谱系。在细胞死亡阻断后也观察到相同的两个异位神经母细胞谱系，并且值得注意的是，在这种情况下产生的异位神经细胞谱系是唇部阳性的。这些发现表明，在野生型大脑的两个特定神经母细胞谱系中，唇部是通过程序性细胞死亡适当终止增殖所必需的。我们对唇功能的分析揭示了该 Hox 基因在胚胎后发育过程中塑造大脑谱系结构中的新细胞自主作用。
The developing brain of Drosophila has become a useful model for studying the molecular genetic mechanisms that give rise to the complex neuronal arrays that characterize higher brains in other animals including mammals. Brain development in Drosophila begins during embryogenesis and continues during a subsequent postembryonic phase. During embryogenesis, the Hox gene labial is expressed in the developing tritocerebrum, and labial loss-of-function has been shown to be associated with a loss of regional neuronal identity and severe patterning defects in this part of the brain. However, nothing is known about the expression and function of labial, or any other Hox gene, during the postembryonic phase of brain development, when the majority of the neurons in the adult brain are generated. Here we report the first analysis of Hox gene action during postembryonic brain development in Drosophila. We show that labial is expressed initially in six larval brain neuroblasts, of which only four give rise to the labial expressing neuroblast lineages present in the late larval brain. Although MARCM-based clonal mutation of labial in these four neuroblast lineages does not result in an obvious phenotype, a striking and unexpected effect of clonal labial loss-of-function does occur during postembryonic brain development, namely the formation of two ectopic neuroblast lineages that are not present in wildtype brains. The same two ectopic neuroblast lineages are also observed following cell death blockage and, significantly, in this case the resulting ectopic lineages are Labial-positive. These findings imply that labial is required in two specific neuroblast lineages of the wildtype brain for the appropriate termination of proliferation through programmed cell death. Our analysis of labial function reveals a novel cell autonomous role of this Hox gene in shaping the lineage architecture of the brain during postembryonic development.
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影响因子：
10.5
作者：
DIEDERICH, RJ;MERRILL, VKL;KAUFMAN, TC
通讯作者：
KAUFMAN, TC
影响因子：
4
作者：
Albertson, R;Chabu, C;Doe, CQ
通讯作者：
Doe, CQ
影响因子：
10.5
作者：
LUO, LQ;LIAO, YJ;JAN, YN
通讯作者：
JAN, YN
影响因子：
16.2
作者：
Bello, BC;Hirth, F;Gould, AP
通讯作者：
Gould, AP
影响因子：
64.5
作者：
Maurange, Cedric;Cheng, Louise;Gould, Alex P.
通讯作者：
Gould, Alex P.