Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b.
Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b.
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补体片段 C3b/C4b 人类受体的部分 cDNA 克隆的鉴定。
DOI：
10.1073/pnas.82.22.7711
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发表时间：
1985
影响因子：
11.1
通讯作者：
Fearon,DT
中科院分区：
文献类型：
--
作者：
Wong,WW;Klickstein,LB;Smith,JA;Weis,JH;Fearon,DT
从纯化的人补体C3b/C4b受体(CR1)出发，根据胰蛋白酶多肽的氨基酸序列合成冗余寡核苷酸。这些探针被用来筛选大小选择的人扁桃体cDNA文库。鉴定出1个与3个寡核苷酸探针杂交的阳性克隆。该插入片段长度为1.5kb，包含与寡核苷酸探针同源的序列以及与另一个独立的CR1胰酶多肽对应的核苷酸序列。以该插入片段为探针的印迹杂交分析显示，人扁桃体中的CR1信息有两种不同的类型，分别为9和11个碱基。CR1插入片段的两个EcoRI片段相互杂交，提示存在同源序列。当作为探针用于人类DNA的Southern印迹分析时，每个片段都识别出相似但不相同的多个限制性片段模式，表明单个CR1基因中存在一系列同源结构域或存在多个CR1基因。此外，在一个家系中还发现了一个BamHI片段，该片段与CR1的S异型蛋白的表达相分离。因此，CR1蛋白多态变体的分子量差异是基于核苷酸序列的差异。
Redundant oligonucleotides were synthesized based on amino acid sequences of tryptic peptides from the purified receptor for human complement fragments C3b/C4b (CR1). These probes were used to screen a size-selected human tonsilar cDNA library. A single positive clone was identified that hybridized to three oligonucleotide probes. The cDNA insert was 1.5 kilobases in length and contained sequences homologous to those of the oligonucleotide probes as well as nucleotide sequences corresponding to another independent CR1 tryptic peptide. Blot-hybridization analysis using fragments of the cDNA insert as probes revealed two distinct species of the CR1 message of 9 and 11 kilobases in human tonsil mRNA. The two EcoRI fragments of the CR1 cDNA insert hybridized to each other, suggesting the presence of homologous sequences. When used as probes in Southern blot analysis of human DNA, each fragment identified similar but not identical patterns of multiple restriction fragments, indicating either a series of homologous domains in a single CR1 gene or the presence of multiple CR1 genes. Furthermore, an additional BamHI fragment was found to segregate with the expression of the S allotype of the CR1 protein in a family. Thus, the molecular weight difference in the polymorphic variants of the CR1 protein is based on differences in nucleotide sequences.
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通过与 C4 和 Slp 特异性 cDNA 杂交鉴定出鼠第四补体成分 (C4) 和性别限制蛋白 (Slp) 的基因。
DOI：
10.1073/pnas.81.15.4908
发表时间：
1984
影响因子：
11.1
作者：
Ogata,RT;Sepich,DS
通讯作者：
Sepich,DS
C3b/C4b 受体 (CR1) 的多态性：第四个等位基因的表征。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dykman,TR;Hatch,JA;Aqua,MS;Atkinson,JP
通讯作者：
Atkinson,JP
DOI：
10.1016/s0021-9258(17)44608-4
发表时间：
1983-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Spiegelman;M. Frank;H. Green
通讯作者：
B. Spiegelman;M. Frank;H. Green
DOI：
10.1073/pnas.80.6.1698
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DYKMAN, TR;COLE, JL;ATKINSON, JP
通讯作者：
ATKINSON, JP
影响因子：
4.1
作者：
H. Bennett;A. Hudson;C. McMartin;G. Purdon
通讯作者：
G. Purdon