Environmental and nutritional factors that affect growth and metabolism of the pneumococcal serotype 2 strain D39 and its nonencapsulated derivative strain R6.
Environmental and nutritional factors that affect growth and metabolism of the pneumococcal serotype 2 strain D39 and its nonencapsulated derivative strain R6.
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DOI：
10.1371/journal.pone.0058492
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Neves AR
中科院分区：
文献类型：
--
作者：
Carvalho SM;Kuipers OP;Neves AR
在肺炎链球菌中碳水化合物代谢和毒力之间的联系已经被反复建立。为了进一步研究这些联系，我们开发了一种化学成分确定的培养基（CDM）和标准化的生长条件，允许相关肺炎球菌菌株D39和R6的高生长产量。利用确定的培养基能够在pH、温度和气体气氛的受控条件下评价不同的环境和营养因素对生长和发酵模式的影响。相同的生长条件对未包封的R6及其包封的祖细胞D39的影响不同。半好氧气氛和尿嘧啶（D39胶囊的基本组分）浓度升高，显著提高了D39的生长速率和生物量。相比之下，在菌株R6中，严格的厌氧条件可以提高生长速率，并且尿嘧啶对生物量没有影响。在氧气的存在下，生长速率的差异主要归因于菌株D39中丙酮酸氧化酶的较低活性。我们的数据表明菌株D39中的胶囊生产和尿嘧啶可用性之间存在复杂的联系。在本研究中，我们还成功地应用体内核磁共振技术研究了糖代谢的S。pneumoniae R6.通过13C-NMR在线监测葡萄糖消耗、终产物形成和细胞内代谢物库的演变。此外，在葡萄糖脉冲后，对NTP和无机磷酸盐池进行31P-NMR。这些结果代表了第一个非侵入性获得的S代谢谱数据。pneumoniae，并为更好地理解中枢代谢的调节铺平道路。
Links between carbohydrate metabolism and virulence in Streptococcus pneumoniae have been recurrently established. To investigate these links further we developed a chemically defined medium (CDM) and standardized growth conditions that allowed for high growth yields of the related pneumococcal strains D39 and R6. The utilization of the defined medium enabled the evaluation of different environmental and nutritional factors on growth and fermentation patterns under controlled conditions of pH, temperature and gas atmosphere. The same growth conditions impacted differently on the nonencapsulated R6, and its encapsulated progenitor D39. A semi-aerobic atmosphere and a raised concentration of uracil, a fundamental component of the D39 capsule, improved considerably D39 growth rate and biomass. In contrast, in strain R6, the growth rate was enhanced by strictly anaerobic conditions and uracil had no effect on biomass. In the presence of oxygen, the difference in the growth rates was mainly attributed to a lower activity of pyruvate oxidase in strain D39. Our data indicate an intricate connection between capsule production in strain D39 and uracil availability. In this study, we have also successfully applied the in vivo NMR technique to study sugar metabolism in S. pneumoniae R6. Glucose consumption, end-products formation and evolution of intracellular metabolite pools were monitored online by 13C-NMR. Additionally, the pools of NTP and inorganic phosphate were followed by 31P-NMR after a pulse of glucose. These results represent the first metabolic profiling data obtained non-invasively for S. pneumoniae, and pave the way to a better understanding of regulation of central metabolism.
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DOI：
10.1111/j.1365-2672.1995.tb03131.x
发表时间：
1995-07-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
COCAIGNBOUSQUET, M;GARRIGUES, C;LOUBIERE, P
通讯作者：
LOUBIERE, P
影响因子：
2.8
作者：
Gonçalves, VMM;Takagi, M;Tanizaki, MM
通讯作者：
Tanizaki, MM
影响因子：
7
作者：
Griffin, TJ;Gygi, SP;Aebersold, R
通讯作者：
Aebersold, R
影响因子：
2.8
作者：
Kloosterman, TG;Bijlsma, JJE;Kuipers, OP
通讯作者：
Kuipers, OP
研究肺炎球菌类型的诱导物质转化的化学性质：通过从III型肺炎球菌分离出的脱氧核糖核酸分数诱导转化。
DOI：
10.1084/jem.79.2.137
发表时间：
1944-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Avery OT;Macleod CM;McCarty M
通讯作者：
McCarty M