Effects of in ovo feeding of t10,c12-conjugated linoleic acid on hepatic lipid metabolism and subcutaneous adipose tissue deposition in newly hatched broiler chicks.
Effects of in ovo feeding of t10,c12-conjugated linoleic acid on hepatic lipid metabolism and subcutaneous adipose tissue deposition in newly hatched broiler chicks.
复制标题
蛋内饲喂t10,c12共轭亚油酸对初孵肉鸡肝脏脂质代谢及皮下脂肪组织沉积的影响
DOI：
10.1016/j.psj.2022.101797
复制
发表时间：
2022-05
期刊：
影响因子：
4.4
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Xu W;Song Z;Wang W;Li X;Yan P;Shi T;Fu C;Liu X
本研究旨在探讨卵内饲喂t10，c12-共轭亚油酸（CLA）是否能调节雏鸡肝脏脂质代谢，减少肝脏脂质蓄积。选择360枚无特定致病菌的受精卵，随机分为6组。在胚胎孵化第11天（E11），将0、1.5、3.0、4.5、6.0或7.5 mg t10、c12-CLA注射到卵中。结果表明，t10，c12-CLA能显著降低雏鸡皮下脂肪组织（SAT）质量和SAT相对重量，且呈线性和二次曲线关系（P < 0.05）;在肝脏中，随着t10，c12-CLA剂量的增加，甘油三酯水平呈线性和二次曲线下降，总胆固醇呈二次曲线下降（P < 0.05）。与此同时，t10、c12-CLA组大鼠肝组织中肉毒碱棕榈酰转移酶-1a（CPT 1a）含量和多不饱和脂肪酸比例呈二次曲线上升（P < 0.05），丙二醛含量下降，谷胱甘肽过氧化物酶和总抗氧化能力活性升高（P < 0.05）。此外，卵内饲喂t10，c12-CLA以线性和二次曲线方式降低脂肪酸合成酶、乙酰辅酶A羧化酶1的mRNA表达水平（P < 0.05），显著降低肝脏脂肪三酰甘油脂肪酶和硬脂酰辅酶A去饱和酶mRNA的表达（P < 0.05），同时呈二次曲线上调CPT 1a mRNA表达，呈线性和二次曲线上调AMP活化蛋白激酶α mRNA表达（P < 0.05）。随着t10，c12-CLA剂量的增加，SAT中过氧化物酶体增殖物激活受体γ（PPARγ）和固醇调节元件结合蛋白1c的mRNA表达呈线性和二次曲线下降（P < 0.05），而PPARα和CPT 1a的mRNA表达呈线性和二次曲线上升（P < 0.05）。总之，我们的研究结果表明，在卵内喂养的t10，c12-CLA通过抑制脂肪酸的合成和刺激肝脏中的脂解和抑制皮下脂肪组织中的脂肪细胞分化来抑制新孵化的小鸡中的脂质积累。
The purpose of this study was to investigate whether in ovo feeding of t10,c12-conjugated linoleic acid (CLA) could regulate hepatic lipid metabolism and decrease lipid accumulation in newly hatched chicks. Three hundred and sixty fertilely specific pathogen-free hatching eggs were selected and randomly divided into 6 groups. On embryonic day 11 of incubation (E11), 0, 1.5, 3.0, 4.5, 6.0, or 7.5 mg t10,c12-CLA were injected into the eggs. The results indicated that in ovo feeding of t10,c12-CLA significantly decreased the subcutaneous adipose tissue (SAT) mass and the relative SAT weight of newly hatched chicks in linear and quadratic manners (P < 0.05). In liver, the levels of triglycerides were reduced linearly and quadratically and total cholesterol were reduced quadratically as the dose of t10,c12-CLA increased (P < 0.05). Meanwhile, the hepatic carnitine palmitoyltransferase-1a (CPT1a) content and polyunsaturated fatty acid proportion were increased quadratically in t10,c12-CLA groups (P < 0.05), accompanied by the decrease of malondialdehyde level and the increase of glutathione peroxidase and total antioxidant capacity activities (P < 0.05). In addition, in ovo feeding of t10,c12-CLA decreased the mRNA expression levels of fatty acid synthase, acetyl-CoA carboxylase 1 in linear and quadratic manners (P < 0.05), and decreased the mRNA expression of adipose triacylglyceride lipase and stearoyl-CoA desaturase significantly in liver (P < 0.05), accompanied by upregulating the mRNA expression of CPT1a quadratically and AMP-activated protein kinase α linearly and quadratically (P < 0.05). In SAT, the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ) and sterol regulatory element-binding protein-1c were decreased linearly and quadratically (P < 0.05), and the expression of PPARα and CPT1a genes were increased linearly and quadratically as the dose of t10,c12-CLA increased (P < 0.05). In conclusion, our findings demonstrate that in ovo feeding of t10,c12-CLA alleviates lipid accumulation in newly hatched chicks by suppressing fatty acid synthesis and stimulating lipolysis in the liver and inhibiting adipocyte differentiation in subcutaneous adipose tissue.
登录
查看更多内容
影响因子：
--
作者：
Maslak E;Buczek E;Szumny A;Szczepnski W;Franczyk-Zarow M;Kopec A;Chlopicki S;Leszczynska T;Kostogrys RB
通讯作者：
Kostogrys RB
影响因子：
1.9
作者：
Evans, M;Geigerman, C;McIntosh, M
通讯作者：
McIntosh, M
影响因子：
7
作者：
Dai, Dong;Wu, Shu-geng;Wang, Jing
通讯作者：
Wang, Jing
DOI：
10.1073/pnas.0404297101
发表时间：
2004-08-03
影响因子：
11.1
作者：
Chen, GX;Liang, GS;Brown, MS
通讯作者：
Brown, MS
DOI：
10.1111/j.1749-6632.2010.05798.x
发表时间：
2010-01-01
期刊：
YEAR IN DIABETES AND OBESITY
影响因子：
--
作者：
Fernandez-Twinn, Denise S.;Ozanne, Susan E.
通讯作者：
Ozanne, Susan E.