Expression of pathogenesis-related genes in cotton roots in response to Verticillium dahliae PAMP molecules
Expression of pathogenesis-related genes in cotton roots in response to Verticillium dahliae PAMP molecules
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棉花根部病程相关基因响应黄萎病 PAMP 分子的表达
DOI：
10.1007/s11427-017-9071-9
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发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Hua Chenlei
中科院分区：
文献类型：
--
作者：
Du Xuan;Wang Sheng;Gao Feng;Zhang Lisha;Zhao Jian-Hua;Guo Hui-Shan;Hua Chenlei
黄萎病已成为许多重要经济作物的主要威胁。由于缺乏标记基因，棉花与黄萎病菌互作过程中植物免疫是否发生以及如何发生尚不清楚。利用棉花基因组发现了病程相关基因家族，这些基因家族已被广泛用作植物免疫反应的标记。对G.为了研究棉花PR基因在植物免疫过程中的作用，我们用两种免疫原性肽flg 22和nlp 20以及与nlp 20高度同源的三种棉花黄萎病菌衍生肽nlp 20 Vd 2、nlp 23 Vd 3和nlp 23 Vd 4处理棉花根。实时荧光定量PCR结果显示，14 G. hirsutum PR基因（GhPR）家族的诱导或抑制独立地响应flg 22，nlp 20，nlp 20 Vd 2，nlp 23 Vd 3，和nlp 23 Vd 4。大多数GhPR基因在免疫原性肽孵育后3小时表达最高。与flg 22和nlp 20相比，nlp 20 Vd 2更有效地触发GhPR基因的上调表达。值得注意的是，nlp 23 Vd 3和nlp 23 Vd 4都能够诱导GhPR基因上调，尽管它们不诱导棉花叶片坏死。因此，我们的研究结果提供了标记基因和新的免疫原性肽，为进一步研究棉花-V. dahliae相互作用。
Verticillium wilt disease becomes a major threat to many economically important crops. It is unclear whether and how plant immunity takes place during cotton-Verticillium interaction due to the lack of marker genes. Taking advantage of cotton (Gossypium hirsutum) genome, we discovered pathogenesis-related (PR) gene families, which have been widely used as markers of immune responses in plants. To profile the expression of G. hirsutum PR genes in the process of plant immunity, we treated cotton roots with two immunogenic peptides, flg22 and nlp20 known as pathogen-associated molecular patterns, as well as three Verticillium dahliae-derived peptides, nlp20Vd2, nlp23Vd3, and nlp23Vd4which are highly identical to nlp20. Quantitative real-time PCR results revealed that 14 G. hirsutum PR gene (GhPR) families were induced or suppressed independently in response to flg22, nlp20, nlp20Vd2, nlp23Vd3, and nlp23Vd4. Most GhPR genes are expressed highest at 3 h post incubation of immunogenic peptides. Compared to flg22 and nlp20, nlp20Vd2is more effective to trigger up-regulated expression of GhPR genes. Notably, both nlp23Vd3and nlp23Vd4are able to induce GhPR gene up-regulation, although they do not induce necrosis on cotton leaves. Thus, our results provide marker genes and new immunogenic peptides for further investigation of cotton-V. dahliae interaction.
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影响因子：
5.3
作者：
Kouzai Y;Kimura M;Yamanaka Y;Watanabe M;Matsui H;Yamamoto M;Ichinose Y;Toyoda K;Onda Y;Mochida K;Noutoshi Y
通讯作者：
Noutoshi Y
影响因子：
2.7
作者：
H. G. M. C. Fadden;R. Chapple;R. Feyter;E. Dennis
通讯作者：
H. G. M. C. Fadden;R. Chapple;R. Feyter;E. Dennis
DOI：
10.1002/chin.200652241
发表时间：
2006-12
期刊：
ChemInform
影响因子：
--
作者：
M. Gijzen;T. Nuernberger
通讯作者：
M. Gijzen;T. Nuernberger
影响因子：
46.9
作者：
Li, Fuguang;Fan, Guangyi;Yu, Shuxun
通讯作者：
Yu, Shuxun
影响因子：
4.9
作者：
Niki, T;Mitsuhara, I;Ohashi, Y
通讯作者：
Ohashi, Y