Specificity of Immunoglobulin Response to Nontuberculous Mycobacteria Infection in People with Cystic Fibrosis.
Specificity of Immunoglobulin Response to Nontuberculous Mycobacteria Infection in People with Cystic Fibrosis.
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DOI：
10.1128/spectrum.01874-22
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发表时间：
2022-08-31
影响因子：
3.7
通讯作者：
Nick, Jerry A.
中科院分区：
文献类型：
--
作者：
Malcolm, Kenneth C.;Wheeler, Emily A.;Calhoun, Kara;Lenhart-Pendergrass, Patricia M.;Rysavy, Noel;Poch, Katie R.;Caceres, Silvia M.;Saavedra, Milene T.;Nick, Jerry A.
非结核分枝杆菌（NTM）感染在慢性肺部疾病中越来越普遍，包括囊性纤维化（CF）。由于相对较大的毒力和固有的抗菌素耐药性，脓肿分枝杆菌是特别关注的。气道培养鉴定是检测肺部感染的标准方法，但由于灵敏度低、培养时间长、依赖痰液或灌洗而受到阻碍。用于检测NTM感染的培养独立试验可以补充或替代痰培养，由于CF （pwCF）患者在高效调节剂治疗下痰量减少，痰培养越来越难以获得。我们描述了一种检测血浆抗m抗体的方法。pwCF对脓疡分枝杆菌裂解物抗原的脓疡抗体。Anti-M。脓疡分枝杆菌IgG和IgA，而非IgM，能够高特异性地区分脓疡分枝杆菌感染与鸟分枝杆菌复合体感染、远端感染或无NTM感染。IgG3亚型占主导地位，其他亚型贡献较小。血浆IgG均能识别水溶性和有机可溶性抗原。一个检测IgG和IgG3的验证队列确定了脓肿分枝杆菌阳性受试者，IgG升高持续数年。这些研究表明，脓肿分枝杆菌细胞裂解物检测CF和脓肿分枝杆菌感染受试者血浆IgG的益处。亚类分析表明，在这些慢性细菌感染患者中，IgG3是主要亚型，这表明在类成熟过程中存在缺陷。作为辅助或替代培养，血清诊断可用于监测慢性肺部疾病中的脓肿分枝杆菌群感染。非结核分枝杆菌（NTM）肺部感染，特别是脓肿分枝杆菌（一种具有高抗生素耐药性的病原体），由于临床结果不佳和在囊性纤维化和其他疾病患者中具有挑战性的检测，引起了人们的高度关注。标准的检测方法是不敏感的，而且越来越困难。我们描述了从血浆中检测ntm特异性抗体来识别感染脓肿分枝杆菌的受试者。该分析是敏感的，并提供了对NTM感染的免疫反应的信息。该检测可用于帮助识别NTM肺部感染的受试者并跟踪疾病进展，无论是单独还是与其他检测相结合。
Nontuberculous mycobacteria (NTM) infections are increasingly prevalent in chronic lung diseases, including cystic fibrosis (CF). Mycobacterium abscessus is of particular concern due to relatively greater virulence and intrinsic antimicrobial resistance. Airway culture identification, the standard method for detecting pulmonary infection, is hindered by low sensitivity, long culture times, and reliance on sputum production or lavage. A culture-independent test for detecting NTM infection could complement, or replace, sputum culture, which is becoming more difficult to obtain with reduced sputum production by people with CF (pwCF) on highly effective modulator therapy. We describe an assay for the detection of plasma anti-M. abscessus antibodies of pwCF to antigens from M. abscessus lysates. Anti-M. abscessus IgG and IgA, but not IgM, discriminated with high specificity subjects infected with M. abscessus from those infected by M. avium complex, and from those with distant or no NTM infections. The IgG3 subclass predominated with minor contributions by other subclasses. Both aqueous and organic soluble antigens were recognized by plasma IgG. A validation cohort measuring IgG and IgG3 identified M. abscessus positive subjects, and elevated IgG was sustained over several years. These studies show the benefit of M. abscessus cell lysates to detect plasma IgG of subjects with CF and M. abscessus infections. Subclass analysis suggests that IgG3 is the predominant subtype in these subjects with chronic bacterial infections suggesting a defect in class maturation. Serodiagnosis could be useful to monitor M. abscessus group infections in chronic lung disease as an adjunct or alternative to culture. IMPORTANCE Lung infections with nontuberculous mycobacteria (NTM), and particularly Mycobacterium abscessus, a pathogen with high antibiotic resistance, are of great concern due to poor clinical outcomes and challenging detection in people with cystic fibrosis and other diseases. Standard detection methods are insensitive and increasingly difficult. We describe the measurement of NTM-specific antibodies from plasma to identify subjects infected with M. abscessus. The assay is sensitive and provides information on the immune response to NTM infections. This assay could be used to help identify subjects with NTM pulmonary infections and track disease progression, either alone or in conjunction with other tests.
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影响因子：
7.3
作者：
Collins AM;Jackson KJ
通讯作者：
Jackson KJ
影响因子：
5.2
作者：
Le Moigne, Vincent;Roux, Anne-Laure;Herrmann, Jean-Louis
通讯作者：
Herrmann, Jean-Louis
影响因子：
9.4
作者：
BRETT, MM;GHONEIM, ATM;LITTLEWOOD, JM
通讯作者：
LITTLEWOOD, JM
影响因子：
2.6
作者：
Enomoto, K;Oka, S;Yano, I
通讯作者：
Yano, I
影响因子：
3
作者：
LAGACE, J;PELOQUIN, L;MONTIE, TC
通讯作者：
MONTIE, TC