Multi-Lineage Human Endometrial Organoids on Acellular Amniotic Membrane for Endometrium Regeneration.
Multi-Lineage Human Endometrial Organoids on Acellular Amniotic Membrane for Endometrium Regeneration.
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DOI：
10.1177/09636897231218408
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发表时间：
2023-01
影响因子：
3.3
通讯作者：
Chen, Xiaojun
中科院分区：
文献类型：
--
作者：
Xu, Yuhui;Cai, Shuyan;Wang, Qian;Cheng, Minzhang;Hui, Xianrui;Dzakah, Emmanuel Enoch;Zhao, Bing;Chen, Xiaojun
关键词：
阿谢曼综合征是一种子宫内膜再生障碍，是由于子宫内膜基底层损伤，导致子宫和子宫颈形成疤痕组织。这通常会导致子宫不孕、月经失调和胎盘异常。虽然干细胞疗法在修复受损子宫内膜和预防宫腔粘连方面取得了广泛进展，但该技术在子宫内膜修复中的高效、有效应用仍存在着床率低、衰老快、肿瘤发生风险等问题。本研究通过开发共培养系统来生成包含子宫内膜上皮类器官（EEO）和子宫内膜间充质干细胞（eMSC）的多谱系子宫内膜类器官（MLEO）来解决这些挑战。通过将这些 MLEO 接种在生物相容性支架（人脱细胞羊膜（HAAM））上来研究这些 MLEO 的功效，以创建生物移植补片，随后将其移植到大鼠子宫内膜损伤模型中。结果表明，HAAM 贴片上的 MLEO 促进子宫内膜血管生成、再生并改善妊娠结局。 HAAM 贴片上的 MLEO 可以作为治疗子宫内膜损伤和预防阿什曼综合征的一种有前景的策略。
Asherman’s syndrome is an endometrial regeneration disorder resulting from injury to the endometrial basal layer, causing the formation of scar tissue in the uterus and cervix. This usually leads to uterine infertility, menstrual disorders, and placental abnormalities. While stem cell therapy has shown extensive progress in repairing the damaged endometrium and preventing intrauterine adhesion, issues of low engraftment rates, rapid senescence, and the risk of tumorigenesis remain to be resolved for efficient and effective application of this technology in endometrial repair. This study addressed these challenges by developing a co-culture system to generate multi-lineage endometrial organoids (MLEOs) comprising endometrial epithelium organoids (EEOs) and endometrial mesenchymal stem cells (eMSCs). The efficacy of these MLEOs was investigated by seeding them on a biocompatible scaffold, the human acellular amniotic membrane (HAAM), to create a biological graft patch, which was subsequently transplanted into an injury model of the endometrium in rats. The results indicated that the MLEOs on the HAAM patch facilitated endometrial angiogenesis, regeneration, and improved pregnancy outcomes. The MLEOs on the HAAM patch could serve as a promising strategy for treating endometrial injury and preventing Asherman’s syndrome.
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