Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides.
Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides.
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DOI：
10.1016/j.nano.2017.03.015
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Jin L;Ding M;Oklopcic A;Aghdasi B;Xiao L;Li Z;Jevtovic-Todorovic V;Li X
本研究旨在评价抗氧化剂纳米颗粒富勒醇在小鼠神经根病和背根神经节（DRG）培养模型中的镇痛作用。椎间盘退变引起明显的痛觉过敏和神经炎症。当椎间盘材料在植入前浸泡在10或100µM富勒醇中时，富勒醇可抵消疼痛致敏和炎症反应。免疫组化显示各组间植入椎间盘材料周围有大量IBA 1阳性巨噬细胞浸润，但富勒醇治疗组IL-1β和IL-6表达降低。在DRG外植体培养中，经TNF-α处理后，IL-1β、NLRP 3和caspase 1的表达显著增加，但加入富勒醇可逆转这种情况。此外，富勒醇还能降低培养的DRG中P物质和CGRP的表达。纳米富勒醇颗粒有效地抵消了疼痛敏化和椎间盘退变引起的炎症级联反应。椎间盘材料引起了显着的痛觉过敏和神经炎症，这是抵消富勒醇管理。各组间巨噬细胞浸润相似，但富勒醇处理组IL-1β和IL-6表达降低。在DRG外植体培养中，经TNF-α处理后，IL-1β、NLRP 3和caspase 1的表达显著增加，但加入富勒醇可逆转。此外，富勒醇还能降低培养的DRG中P物质和CGRP的表达。
The present study aimed to evaluate the analgesic effect of the antioxidant nanoparticle fullerol in a mouse radiculopathy and a dorsal root ganglion (DRG) culture models. Intervertebral disc degeneration causes significant hyperalgesia and nerve inflammation. Pain sensitization and inflammatory reaction were counteracted by fullerol when disc material was bathed in 10 or 100µM of fullerol prior to implantation. Immunohistochemistry showed similar massive IBA1 positive macrophage infiltration surrounding implanted disc material among groups, but IL-1β and IL-6 expression was decreased in fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but this was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs. Nanoparticle fullerol effectively counteracts pain sensitization and the inflammatory cascade caused by disc degeneration. Intervertebral disc material caused a significant hyperalgesia and nerve inflammation, which were counteracted by fullerol administration. Similar massive macrophage infiltration was observed among groups, but IL-1β and IL-6 expression was decreased in fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs.
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影响因子：
3.7
作者：
Jin L;Liu Q;Scott P;Zhang D;Shen F;Balian G;Li X
通讯作者：
Li X
影响因子：
2.9
作者：
Liu Q;Cui Q;Li XJ;Jin L
通讯作者：
Jin L
DOI：
10.3791/51212
发表时间：
2013-12-19
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Martinov T;Mack M;Sykes A;Chatterjea D
通讯作者：
Chatterjea D
影响因子：
4.9
作者：
Le Maitre, Christine Lyn;Hoyland, Judith Alison;Freemont, Anthony J
通讯作者：
Freemont, Anthony J
DOI：
10.1196/annals.1397.069
发表时间：
2007-01-01
期刊：
SIGNAL TRANSDUCTION PATHWAYS, PT D
影响因子：
--
作者：
Bachmeier, Beatrice E.;Nerlich, Andreas G.;Boos, Norbert
通讯作者：
Boos, Norbert