In vivo wide-area cellular imaging by side-view endomicroscopy.
In vivo wide-area cellular imaging by side-view endomicroscopy.
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DOI:
10.1038/nmeth.1440
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发表时间:
2010-04
期刊:
影响因子:
48
通讯作者:
Yun, Seok H.
中科院分区:
文献类型:
--
作者:
Kim, Pilhan;Chung, Euiheon;Yamashita, Hiroshi;Hung, Kenneth E.;Mizoguchi, Atsushi;Kucherlapati, Raju;Fukumura, Dai;Jain, Rakesh K.;Yun, Seok H.
In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts and may be extended to imaging organ parenchyma such as cerebral cortex in mice. We monitored cell infiltration, vascular changes and tumor progression during inflammation and tumorigenesis in colon over several months.
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