Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage.
Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage.
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DOI：
10.1016/j.celrep.2021.108735
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发表时间：
2021-02-09
期刊：
影响因子：
8.8
通讯作者：
Alberio R
中科院分区：
文献类型：
--
作者：
Zhu Q;Sang F;Withey S;Tang W;Dietmann S;Klisch D;Ramos-Ibeas P;Zhang H;Requena CE;Hajkova P;Loose M;Surani MA;Alberio R
由于获得胚胎材料的途径有限，人类生殖系和编程的研究具有挑战性。然而，猪作为一个模型，可能会提供适用于这两个物种的转录网络和表观遗传重编程的见解。在这里，我们表明，在前和早期迁移阶段，猪原始生殖细胞（PGCs）启动大规模的表观基因组重编程，包括DNA去甲基化涉及TET介导的羟基化，并可能，碱基切除修复（BER）。在女性中也存在macroH2A1消耗和增加的H3K27me3以及X染色体再激活（XCR）。与此同时，糖酵解代谢基因的抑制和一些多能性基因的重新表达，如在植入前胚胎中的那些。我们确定了进化上年轻的转座因子和基因编码区，对急性低甲基化性腺PGCs的DNA去甲基化有抵抗力，具有跨代表观遗传的潜力。对猪生殖系的详细了解可能会对人类生殖系生物学的进步做出重大贡献，包括体外配子发生。猪和人类原始生殖细胞的基因表达谱紧密对齐迁移前猪原始生殖细胞经历DNA去甲基化、XCR和组蛋白重塑猪生殖系中DNA去甲基化抗性位点的鉴定Zhu et al.显示猪原始生殖细胞（PGCs）在特化后经历DNA去甲基化、组蛋白重塑和X染色体再活化。猪PGCs在全基因组去甲基化后保留很少的甲基化位点，具有跨代遗传的潜力。种属比较显示猪和人PGCs的转录谱非常相似。
Investigations of the human germline and programming are challenging because of limited access to embryonic material. However, the pig as a model may provide insights into transcriptional network and epigenetic reprogramming applicable to both species. Here we show that, during the pre- and early migratory stages, pig primordial germ cells (PGCs) initiate large-scale epigenomic reprogramming, including DNA demethylation involving TET-mediated hydroxylation and, potentially, base excision repair (BER). There is also macroH2A1 depletion and increased H3K27me3 as well as X chromosome reactivation (XCR) in females. Concomitantly, there is dampening of glycolytic metabolism genes and re-expression of some pluripotency genes like those in preimplantation embryos. We identified evolutionarily young transposable elements and gene coding regions resistant to DNA demethylation in acutely hypomethylated gonadal PGCs, with potential for transgenerational epigenetic inheritance. Detailed insights into the pig germline will likely contribute significantly to advances in human germline biology, including in vitro gametogenesis. Gene expression profiles of pig and human primordial germ cells are closely aligned Pre-migratory pig PGCs undergo DNA demethylation, XCR, and histone remodeling Identification of DNA demethylation-resistant loci in the pig germline Zhu et al. show that pig primordial germ cells (PGCs) undergo DNA demethylation, histone remodeling, and X chromosome reactivation after specification. Pig PGCs retain few methylated loci after genome-wide demethylation, with potential for transgenerational inheritance. Species comparisons shows close similarities in transcriptional profiles of pig and human PGCs.
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DOI：
10.1126/science.1229277
发表时间：
2013-01-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hackett JA;Sengupta R;Zylicz JJ;Murakami K;Lee C;Down TA;Surani MA
通讯作者：
Surani MA
影响因子：
64.5
作者：
Gkountela S;Zhang KX;Shafiq TA;Liao WW;Hargan-Calvopiña J;Chen PY;Clark AT
通讯作者：
Clark AT
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
人类原始生殖细胞的转录组和 DNA 甲基化组景观
影响因子：
64.5
作者：
Guo, Fan;Yan, Liying;Qiao, Jie
通讯作者：
Qiao, Jie